Sequences from confirmation amplicon


Confirmation sequences for line 334D08 and hit T20D1 are shown.
Line ID 334D08
Locus T20D1 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6591681
Primers used for PCR MM39 - 8474, show primer details
   
Sequence Name 57-K019979-022-334-D08C-MM39
GenBank Accession LN509277 [GenBank]
Sequencing primer gene specific primer, MM39: 5'-CATGAGTAATGATTGCGACAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K019979-022-334-D08C-MM39
CAACTCTACAATCTTCTGCCAAAATTCCGTCGCATGCTTATATTTTGGCATTTTAACGTT
TCAACACTTCCTCATCGTGACATCATAATTATAAGAGATTTATGGTAGATATATAGTAAT
TTATATAAACTTCTATTCATATATGTTCTTTAGTGTTTAAGTTTGAAATAAATGTTTAAG
GTACATAACCTGTTCAACTAGTTTTTGAAACTATACTAGTTTACAATTTTCGTCCGATCA
ATGTATATATTTAAAGTATGTTTCTACTATTTTGATTTTTAGGATAAATCATACCAGAGA
TGCTTCCGGACCACGTTTGGACCGGTGGTTTACTTTAGTTTAAGACATTACTTCAACTAT
CTTAGTTGACTTCATGTCCGGGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAA
ATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATC
TTTATTTCGACGTGTCTACATTC
   
Sequence Name 61-K019979-022-334-D08C-8409
GenBank Accession LN510399 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K019979-022-334-D08C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTCCCGGACATGAAGTCAACTAAGATAGTTGA
AGTAATGTCTTAAACTAAAGTAAACCACCGGTCCAAACGTGGTCCGGAAGCATCTCTGGT
ATGATTTATCCTAAAAATCAAAATAGTAGAAACATACTTTAAATATATACATTGATCGGA
CGAAAATTGTAAACTAGTATAGTTTCAAAAACTAGTTGAACAGGTTATGTACCTTAAACA
TTTATTTCAAACTTAAACACTAAAGAACATATATGAATAGAAGTTTATATAAATTACTAT
ATATCTACCATAAATCTCTTATAATTATGATGTCACGATGAGGAAGTGTTGAAACGTTAA
AATGCCAAAATATAAGCATGCGACGGAATTTTGGCAGAAGATTGTAGAGTTGTAATCTGT
CGCAATCATTACTC
Primers used for PCR MM40 - 8474, show primer details
   
Sequence Name 59-K019980-022-334-D08C-MM40
GenBank Accession LN509863 [GenBank]
Sequencing primer gene specific primer, MM40: 5'-TGAAAATATCATAGTCGAGAGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K019980-022-334-D08C-MM40
TGACAAGTGTACATATGTATCTAGCTGTGGATTCCACCAAAATTCTGGCAGGGCCATGAT
CTAAAAACTGAGACTGCGCGTGTTGTTTTGCAGTGATTTGTATTTCATATTTGCACCATC
CTACACAGTCCACTTGGTATCGTAACCAAACATAAGGAGAACCTAATTACATTATTGTTT
TAATTTCGTCAAACTGGTTTTTACCTTTTAGTTACATAGTTGATTCTTCATTTGTTTTAG
TAGTTATGGAGCACAATAATGTGCAACAAAGAAAGATCATAGTGGATTAATATGTTGAGA
GGTCAGAAATTCTTGGTTAACAAAAAAAAAAAAGTTACAAGGACTGAAATTTTGGGGGGG
AAAAAGCCATAGCTTTTAAAACATGATTGAACTTAAAAGTGATGTTATGGTTTGAGGGGA
AAAAGGTTGAAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATG
ATGGCCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
AATGCCCGCAGCGTTTTTATAAAATGAAAGTACTTTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACA
   
Sequence Name 63-K019980-022-334-D08C-8409
GenBank Accession LN510880 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K019980-022-334-D08C-8409
ATTTCCCGGACATGAAGCCATTTACTTCAACCTTTTTCCCCTCAAACCATAACATCACTT
TTAAGTTCAATCATGTTTTAAAAGCTATGGCTTTCTCCCACCCAAAATCTCAGTCCTTGT
AACTTTTTTTTTTTTGTTAACCAAAAATTTCTGACCTCTCAACATATTAATCCACTATGA
TCTTTCTTTGTTGCACATTATTGGGCTCCATAACTACTAAAACAAATGAAGAATCAACTA
TGTAACTAAAAGGTAAAAACCAGTTTGACGAAATTAAAACAATAATGTAATTAGGTTCTC
CTTATGTTTGGTTACAATACCAAGTGGACTGTGTAGGATGGTGCAAATATGAAATACAAA
TCACTGCAAAACAACACGCGCAGTCTCAGTTTTTAAATCATGGCCCTGCCAAAATTTTGG
GGGA

Last Updated on 06.12.2018 16:11