Sequences from confirmation amplicon


Confirmation sequences for line 336D03 and hit At4g30930 are shown.
Line ID 336D03
Locus At4g30930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15051721
Primers used for PCR MM46 - 8474, show primer details
   
Sequence Name 07-K020131-022-336-D03C-MM46
GenBank Accession LN489061 [GenBank]
Sequencing primer gene specific primer, MM46: 5'-TAATACGCATGGTGATTGATTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K020131-022-336-D03C-MM46
AAACATTCAGGAACTACTACCGATGCACTTCAGCGGGATGTCCAGTTCGTAAACACATTG
AGACAGCAGTCGAGAACACAAAAGCAGTAATAATCACATACAAAGGAGTACACAACCACG
ACATGCCGGTGCCTAAGAAACGCCATGGTCCTCCAAGCTCAATGCTCGTAGCTGCAGCCG
CTCCAACATCAATGAGAACCAGGACAGACGATCAGGTGAACATTCCGACTTCAAGCCAGT
GCTCGGTGGGGCGAGAAAGTGAGAAGCAGAGTAAAGAAGCATTGGACGTTGGTGGAGAGA
AAGTGATGGAATCAGCTAGGACTTTGTTGAGCATTGGATTCGAAATCAAGCAATGCTGAT
TATATGTAGTTTATGAGTTTTATAGAGTTCATCGACTCAATTTTCGCCTCCTTGTTCTTG
TTATATAATAATAATAAAAAACCCCATGAAGATTACCAACTAAATACGTTAACATTCGTC
TGTGTAATGATGGTATATGTTTATTGTGATATTAAATCGCTAATTTTAGTGACTTCAATA
TGTCCGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCANATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
   
Sequence Name 08-K020131-022-336-D03C-8409
GenBank Accession LN489527 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K020131-022-336-D03C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCCGGACATATTGAAGTCAC
TAAAATTAGCGATTTAATATCACAATAAACATATACCATCATTACACAGACGAATGTTAA
CGTATTTAGTTGGTAATCTTCATGGGGTTTTTTATTATTATTATATAACAAGAACAAGGA
GGCGAAAATTGAGTCGATGAACTCTATAAAACTCATAAACTACATATAATCAGCATTGCT
TGATTTCGAATCCAATGCTCAACAAAGTCCTAGCTGATTCCATCACTTTCTCTCCACCAA
CGTCCAATGCTTCTTTACTCTGCTTCTCACTTTCTCGCCCCACCGAGCACTGGCTTGAAG
TCGGAATGTTCACCTGATCGTCTGTCCTGGTTCTCATTGATGTTGGAGCGGCTGCAGCTA
CGAGCATTGAGCTTGGAGGACCATGGCGTTTCTTAGGCACCGGCATGTCGTGGTTGTGTA
CTCCTTTGTATGTGATTATTACTGCTTTTGTGTTCTCGACTGCTGTCTCAATGTGTTTAC
GAACTGGACATCCCGCTGAAGTGCATCGGTAGTAGTTCCTGAATGTTTGAAACAGAACAA
ATCAAT
Primers used for PCR MM45 - 8474, show primer details
   
Sequence Name 09-K020131-022-336-D03C-MM45
GenBank Accession LN489977 [GenBank]
Sequencing primer gene specific primer, MM45: 5'-GCTAACTTTAAACTGATGCGAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K020131-022-336-D03C-MM45
ATAAAAATAACCACTCAGCATCAGTGAGAGTGAGAAATGAAACTAAAGGATTAATTGAGA
TAGCACAGAGACGAGACCTGAACAATGGCAAACACTGGTTCATAGGGTTTGAAAAGTCTC
TCCGAAGGTTTAAGAGGACCCATCACCTTGTACCCTATCTCCTCCGCCTCTTCCACTTTC
TCCGCCGGTGAATATTCCCTTTCTACTTCCATATCCGCCGAATCCTCAAAGTCTTCCCCC
TCCTCCTCATCATCATCCTCTCCTTCACTGCTTTCTTCATCTTCATCAGTATCCTTTGTG
TTCGAAGAGAAACAACGATCTTGAGAGCGATACCAATGACTGTACCATGGCAAATTCCTA
GTGAGAGATCGATTCGGAATTACGGTCCCATGAGAAATGCTGGTCCCGGAAAACTCAATC
CCATGAAATGAAGAGATCGATCGCGTCTGGTTGATGGAGAAAACAGTCGTAGCGCGGCGG
CTCAGCTCACGAAAGCATCGAAGACTCGCCATCGGAGAGCACGAACACACTAGGGTTTAC
ACAGAGTAGAGAGAAAGGGTTTTTGTTCTCGTCTTTTAGTCCCTTTCAAGTTTCAGAAGA
CCCATATGGAGATATTGAAAGTCACTAAAATTAGCGATGTCCGGGAAAGGGTTTTTCAGG
ATATATTCAATTGTAAATGGCTTCATGT
   
Sequence Name 10-K020131-022-336-D03C-oriL
GenBank Accession LN490449 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K020131-022-336-D03C-oriL
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAAAACCCTTTCCCGG
ACATCGCTAATTTTAGTGACTTCAATATCTCCATATGGGTCTTCTGAAACTTGAAAGGGA
CTAAAAGACGAGAACAAAAACCCTTTCTCTCTACTCTGTGTAAACCCTAGTGTGTTCGTG
CTCTCCGATGGCGAGTCTTCGATGCTTTCGTGAGCTGAGCCGCCGCGCTACGACTGTTTT
CTCCATCAACCAGACGCGATCGATCTCTTCATTTCATGGGATTGAGTTTTCCGGGACCAG
CATTTCTCATGGGACCGTAATTCCGAATCGATCTCTCACTAGGAATTTGCCATGGTACAG
TCATTGGTATCGCTCTCAAGATCGTTGTTTCTCTTCGAACACAAAGGATACTGATGAAGA
TGAAGAAAGCAGTGAAGGAGAGGATGATGATGAGGAGGAGGGGGAAGACTTTGAGGATTC
GGCGGATATGGAAGTAGAAAGGGAATATTCACCGGCGGAGAAAGTGGAAGAGGCGGAGGA
GATAGGGTACAAGGTGATGGGTCCTCTTAAACCTTCGGAGAGACTTTTCAAACCCTATGA
ACCAGTGTTTGCCATTGTTCAGGTCTCGTCTCTGTGCTATCTCAATTAATCCTTTAGTTT
CATTTCTCACTCTCACTGATGCTGAGTGGTTATTTTTATCCTGTAGATTGGTTCGCAT

Last Updated on 06.12.2018 16:11