Sequences from confirmation amplicon


Confirmation sequences for line 337E01 and hit At3g25730 are shown.
Line ID 337E01
Locus At3g25730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9397596
Primers used for PCR PH56 - 8474, show primer details
   
Sequence Name 11-K017550-022-337-E01C-PH56
GenBank Accession LN490900 [GenBank]
Sequencing primer gene specific primer, PH56: 5'-AGTGTGGAGGTTCCGTTACTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K017550-022-337-E01C-PH56
GGAATAGTAGTCAAAGTTATGTGTTGACCAAAGGTTGGAGTAGATTCGTTAAAGAGAAGA
GACTTTGTGCTGGTGATTTGATCAGTTTTAAAAGATCCAACGATCAAGATCAAAAATTCT
TTATCGGGTGGAAATCGAAATCCGGGTTGGATCTAGAGACGGGTCGGGTTATGAGATTGT
TTGGGGTTGATATTTCTTTAAACGCCGTCGTTGTAGTGAAGGAAACAACGGAGGTGTTAA
TGTCGTCGTTAAGGTGTAAGAAGCAACGAGTTTTGTAATAACAATTTAACAACTTGGGAA
AGAAAAAAAAGCTTTTTGATTTTAATTTCTCTTCAACGTTAATCTTGCTGAGATTATTTA
TGTTGTAAATAACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACAT
   
Sequence Name 12-K017550-022-337-E01C-8409
GenBank Accession LN491357 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K017550-022-337-E01C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGTTATTTACAACATAAATAATCTCAGCA
AGATTAACGTTGAAGAGAAATTAAAATCAAAAAGCTTTTTTTTCTTTCCCAAGTTGTTAA
ATTGTTATTACAAAACTCGTTGCTTCTTACACCTTAACGACGACATTAACACCTCCGTTG
TTTCCTTCACTACAACGACGGCGTTTAAAGAAATATCAACCCCAAACAATCTCATAACCC
GACCCGTCTCTAGATCCAACCCGGATTTCGATTTCCACCCGATAAAGAATTTTTGATCTT
GATCGTTGGATCTTTTAAAACTGATCAAATCACCAGCACAAAGTCTCTTCTCTTTAACGA
AATCAACCCCAACCTTGGGCCAACACTTAATTTTGACAACTATTCCAAAAAAAG

Last Updated on 06.12.2018 16:11