Sequences from confirmation amplicon


Confirmation sequences for line 338F04 and hit At5g47640 are shown.
Line ID 338F04
Locus At5g47640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19309032
Primers used for PCR CK16 - 8474, show primer details
   
Sequence Name 03-K017721-022-338-F04C-CK16
GenBank Accession LN487275 [GenBank]
Sequencing primer gene specific primer, CK16: 5'-CTTTGTTAAGTCCTTGTCCTACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K017721-022-338-F04C-CK16
CTCCACCTCCACTGTCGCTACCGCCACCTGTGGCTCCATACATATGGTTCTGCTGGTGAA
GAAACTGATGATGTTGGTGATACTGCATCCCACCACCACCACCGTAGAACCCACCGCCAT
CTCCGACAGCATCTCTCTGATGCTCTCCGACCTCACCACCAGTCTGTGGCCTCCCTAGTC
CAGTCCTCTCCCCTTCGATCTCCCTAAACCTCTGCAAGTAAACTTTCAATGGCTCAACAT
AATCCTCAAAACCTAGAGTAGTCATAGCCCAGAGCAAATCGTCTCCGTTGATCGTCTTCC
TCTTCTCCTTCTGACACTTATCAGATGCTTCTCCGGTGACGAAGCTGATGAACTCGGAGA
CACACTCCTGCATCGTCTCTTTGGCATCTTTAGAGATCTTGGCGTTGGCGGGCAAGGCCT
TCTTCATGATCCGGCTGACGTTAGCGATCGGCAAGAACCTGTCTTGCTCTCTTGGAGACA
AGGAGGACTGTCCGTTCTGGTTGTTCCCGTTTTGCCCTCCACCGGAATCCCTGTCGGAAT
CCCCCATTTCTGAAAATTACAAGGAATAAAAAAATAAACTTTACTCTTTTTTCAAATCAT
TTCCATCAATGATTCGAAATCGAATTCAATTGGTGGTTGACTTGAGTGGGAAGACTGAAG
CGAGAAAATTTAGGGTTTTAGAAAGAGTAAAAAGAGCAGGTGTGAATTGTTAAGTGTCGG
CTGGTATTGTACACAGTGG
   
Sequence Name 04-K017721-022-338-F04C-8409
GenBank Accession LN487718 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K017721-022-338-F04C-8409
TGATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTCCACAA
CACGTGGCAAAGCCTTAAAGGGCTGTCTATCTCTCAAACAAGATCAATTCCAATCCGACG
GTCGAGCACGTATCCAGTTGTCAGAATCTTATCAGTAAACGGACGGTTCATATAAGATCA
CGTGTCAAGTGAGCAGAAGTCGTGCGTCCTCTCTTTATATAAGCTCCGGCGACTCCTTCA
CCACTGTGTACAATACCAGCCGACACTTAACAATTCACACCTGCTCTTTTTACTCTTTCT
AAAACCCTAAATTTTCTCGCTTCAGTCTTCCCACTCAAGTCAACCACCAATTGAATTCGA
TTTCGAATCATTGATGGAAATGATTTGAAAAAAGAGTAAAGTTTATTTTTTTATTCCTTG
TAATTTTCAGAAATGGGGGATTCCGACAGGGATTCCGGTGGAGGGCAAAACGGGAACAAC
CAGAACGGACAGTCCTCCTTGTCTCCAAGAGAGCAAGACAGGTTCTTGCCGATCGCTAAC
GTCAGCCGGATCATGAAGAAGGCCTTGCCCGCCAACGCCAAGATCTCTAAAGATGCCAAA
GAGACGATGCAGGAGTGTGTCTCCGAGTTCATCAGCTTCGTCACCGGAGAAGCATCTGAT
AAGTGTC

Last Updated on 06.12.2018 16:11