Sequences from confirmation amplicon


Confirmation sequences for line 339G08 and hit T20D1 are shown.
Line ID 339G08
Locus T20D1 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6591855
Primers used for PCR MM41 - 8474, show primer details
   
Sequence Name 43-K019979-022-339-G08C-MM41
GenBank Accession LN504685 [GenBank]
Sequencing primer gene specific primer, MM41: 5'-TCTGTAGACTGCGGAAATTAGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K019979-022-339-G08C-MM41
AAATAATATAACACAATTACTTTGAATTTCGATTCAAAATATTTTAACTATTCGATGTGT
ATTTATTATATATACGATTAAATAAATAAAAAAATTGTAATGTGAAAATATCATAGTCGA
GAGGGGAACTGACAAGTGTACATATGTATCTAGCTGTGGATTCCACCAAAATTCTGGCAG
GGCCATGATCTAAAAACTGAGACTGCGCGTGTTGTTTTGCAGTGATTTGTATTTCATATT
TGCACCATCCTACACAGTCCACTTGGTATCGTAACCAAACATAAGGAGAACCTAATTACA
TTATTGTTTTAATTTCGTCAAACTGGTTTTTACCTTTTAGTTACATAGTTGATTCTTCAT
TTGTTTTAGTAGTTATGGAGCACAAAGAAATTCATATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 47-K019979-022-339-G08C-8409
GenBank Accession LN506051 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K019979-022-339-G08C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATGAATTTCTTTGTGC
TCCATAACTACTAAAACAAATGAAGAATCAACTATGTAACTAAAAGGTAAAAACCAGTTT
GACGAAATTAAAACAATAATGTAATTAGGTTCTCCTTATGTTTGGTTACGATACCAAGTG
GACTGTGTAGGATGGTGCAAATATGAAATACAAATCACTGCAAAACAACACGCGCAGTCT
CAGTTTTTAGATCATGGCCCTGCCAGAATTTTGGTGGAATCCACAGCTAGATACATATGT
ACACTTGTCAGTTCCCCTCTCGACTATGATATTTTCACATTACAATTTTTTTATTTATTT
AATCGTATATATAATAAATACACATCGAATAGTTAAAATATTTTGAATCGAAATTCAAAG
TAATTGTGTTATATTATTTTTTTGACTAATTTCCGC

Last Updated on 06.12.2018 16:11