Sequences from confirmation amplicon


Confirmation sequences for line 339H05 and hit At4g12020 are shown.
Line ID 339H05
Locus At4g12020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7205333
Primers used for PCR IS38 - 8474, show primer details
   
Sequence Name 34-K018976-022-339-H05C-IS38
GenBank Accession LN501229 [GenBank]
Sequencing primer gene specific primer, IS38: 5'-GAAATAACTCAGATGCCTGGCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K018976-022-339-H05C-IS38
TTGACAGATAAGTACGTGATCCCTGGGTTCTAAATGTAGAAAGTCTTTGGTTTTAGAGAA
TTGAATAAATTTTTATTTGGCAAAGTAAGGCTATAGAAGATTAGAAGTTTATTATACTAT
CCTCTCTTTCGTCTGATGTTTGTATTGTCTACTTTATTCTTAAAATCTCGCAGGTCTGAA
TCTGAACTTATAGATGAGATTGTAAGAGATGCTTTAAAGGTGCTATGTTCTGCTGATAAG
GTGAACATGATTGGGATGGATATGCAAGTAGAGGAGATTTTGTCACTGCTATGCATTGAG
TCCCTTGATGTTCGCAGCATTGGTATATGGGGTACAGTTGGTATAGGAAAAACAACCATT
GCTGAAGAGATCTTTCGCAAAATCTCTGTCCAATATGAGACCTGTGTCGTCCTTAAGGAC
CTCCACAAAGAAGTTGAGGTAAAAGGTCACGATGCTGTGAGAGAGAATTTTCTGTCTGAA
GTTTTAGAGGTAGAACCTCATGTTATCCGGATATCTGACATTAAAACAAGCTTCTTGAGA
AGTCGGCTTCAGCGTAAAAGGATCCTTGTTATTCTTGACGATGTGAATGATTACAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
   
Sequence Name 38-K018976-022-339-H05C-8409
GenBank Accession LN502822 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K018976-022-339-H05C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTAATCATTCACA
TCGTCAAGAATAACAAGGATCCTTTTACGCTGAAGCCGACTTCTCAAGAAGCTTGTTTTA
ATGTCAGATATCCGGATAACATGAGGTTCTACCTCTAAAACTTCAGACAGAAAATTCTCT
CTCACAGCATCGTGACCTTTTACCTCAACTTCTTTGTGGAGGTCCTTAAGGACGACACAG
GTCTCATATTGGACAGAGATTTTGCGAAAGATCTCTTCAGCAATGGTTGTTTTTCCTATA
CCAACTGTACCCCATATACCAATGCTGCGAACATCAAGGGACTCAATGCATAGCAGTGAC
AAAATCTCCTCTACTTGCATATCCATCCCAATCATGTTCACCTTATCAGCAGAACATAGC
ACCTTTAAAGCATCTCTTACAATCTCATCTATAAGTTCAGATTCAGACCTGCGAGATTTT
AAGAATAAAGTAGACAATACAAACATCAGACGAAAGAGAGGATAGTATAATAAACTTCTA
ATCTTCTATAGCCTTACTTTGCCAAATAAAAATTTATTCAATTCTCTAAAACCAAAGACT
TTCTACATTTAGAACCCAGGGATCACGTACTTATCTGTCAATGTGTAGCCAGGC

Last Updated on 06.12.2018 16:11