Sequences from confirmation amplicon


Confirmation sequences for line 339H06 and hit At3g53520 are shown.
Line ID 339H06
Locus At3g53520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19844170
Primers used for PCR MP27 - 8474, show primer details
   
Sequence Name 49-K020436-022-339-H06C-8409
GenBank Accession LN506689 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K020436-022-339-H06C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAAGAAAAAACA
GAGAGAACAAAAAGACTTAGCAGAGCTCTTGAAAAAGAGACTATACATATATCAAATCAA
AGCAGAAGAAGGATTATACTCTATATCTCTGTGTGACTGACTTAGAGACCTTTACCTTCG
TCTTCGTTTAAGATTCGGTTACGGAAGTCACTAACCATGCGAGGCAGTCCCTCCCTCAGA
GATATCTTTGGTTCCCAGTTCAACTGTTCCTTTGCTTTGCTTATGTCTGGTTTCCTCTTG
TGAGGATCGTCTGCTGTGTTTGGTTTGAACTCTATGGTCGCGCTTGGGTCGATCACCTCC
TTAACCACCTGCACACAAAGTTCCCATTTCATACACGGAATTGGCGTTTCTTTATGAGAC
ATCTTAAACGTTGATGCAGATTTCTAAGGATGTATATAACAACCATATCAAACTGAATTT
CTTCAAACTAAAACCGACCCAAACTTTCTTTAGATGAAAAGATAAAAACAATTCGAAGTT
TGGACAGACTCTTCACAAAAGCTAAACCAAACCGAGAACTGAATGACCAGGCCTTAACTT
TACCATTTGGGTTTCATATCTCTTTTGCAGGAAGTAAAACGAAGATGGTAAAAGTAAAGC
AAACCTCTGCAAGTTCAAGCATTGTGAATTCTCCGGGTTACCAAGGTTGAAGGGTCCTAC
ATGATCG
   
Sequence Name 50-K020436-022-339-H06C-MP27
GenBank Accession LN507033 [GenBank]
Sequencing primer gene specific primer, MP27: 5'-TGTAGCTTTAATGGAAAACGATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K020436-022-339-H06C-MP27
CCCTTCAACCTTGGTAACCCTGGAGAATTCACAATGCTTGAACTTGCAGAGGTTTGCTTT
ACTTTTACCATCTTCGTTTTACTTCCTGCAAAAGAGATATGAAACCCAAATGGTAAAGTT
AAGGCCTGGTCATTCAGTTCTCGGTTTGGTTTAGCTTTTGTGAAGAGTCTGTCCAAACTT
CGAATTGTTTTTATCTTTTCATCTAAAGAAAGTTTGGGTCGGTTTTAGTTTGAAGAAATT
CAGTTTGATATGGTTGTTATATACATCCTTAGAAATCTGCATCAACGTTTAAGATGTCTC
ATAAAGAAACGCCAATTCCGTGTATGAAATGGGAACTTTGTGTGCAGGTGGTTAAGGAGG
TGATCGACCCAAGCGCGACCATAGAGTTCAAACCAAACACAGCAGACGATCCTCACAAGA
GGAAACCAGACATAAGCAAAGCAAAGGAACAGTTGAACTGGGAACCAAAGATATCTCTGA
GGGAGGGACTGCCTCGCATGGTTAGTGACTTCCGTAACCGAATCTTAAACGAAGACGAAG
GTAAAGGTCTCTAAGTCAGTCACACAGAGATATAGAGTATAATCCTTCTTCTGCTTTGAT
TTGATATATGTATAGTCTCTTTTTCAAGAGCTCTGCTAAGTCTTTTTGTTCTCTCTGTTT
TTTCTTTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAACTATGGAGAACAAGAAAGAGTAATTACCACTTTTTT

Last Updated on 06.12.2018 16:11