Sequences from confirmation amplicon


Confirmation sequences for line 340G06 and hit At3g21350 are shown.
Line ID 340G06
Locus At3g21350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7516986
Primers used for PCR AH34 - 8474, show primer details
   
Sequence Name 33-K105566-0022-340-G06C-AH34
GenBank Accession LN500996 [GenBank]
Sequencing primer gene specific primer, AH34: 5'-AGGTTTGGTTCAGTAGCATCAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K105566-0022-340-G06C-AH34
TAATCACATGGGTTTCAATCAAACATTGTCACTAAATTCTCCACCTAAATCTATCTCCAT
TTCTCTAACAGTATCCCAGGTGAGACCAAACCCTAATTCGACCTAAAGTTGAAACCTTTA
TGAGTTTCAACATAACCCTATCTGTAGAGCAGTGAGGAGGAGCTTACGAGAGGTGAGAGA
GATCGAGAGGGTGAATGGATCTTCTCCGAAGAATCTCATTGTTGCAAGTTGTATCGTAGA
AAGGAGACAAAGCGAAGTAATCGAAGATGTAGTTACGATCAAGAGGGTATGAATTGATCC
AGAGCTGATCCCTGAAGGAAATTCCAGTCATATCCGTTCCCGGAGGCTGTAACGGAGGAG
GAATTTGATCCGCCGCATTGCCGTTGAAGGTATCGGCCGTAGCCGCCGAGAGCAGAGAGG
AATCCATGGAAGTGAGTCACTTCGCCGTGAAGGAGAAGAGCACAGAGAAAGAAAGAGAGC
GAGAGAGAGAATAATATGGTTCGGCCCATTATGTAATTTGGGCTTTCGAATTTCATTGGC
CCAAAATTGAAGGAATTAGACAAATTATAATATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 65-K105567w-0022-340-G06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K105567w-0022-340-G06C-8409
TTCATTTTGGGCCAATGAAATTCGAAAGCCCAAATTACATAATGGGCCGAACCATATTAT
TCTCTCTCTCGCTCTCTTTCTTTCTCTGTGCTCTTCTCCTTCACGGCGAAGTGACTCACT
TCCATGGATTCCTCTCTGCTCTCGGCGGCTACGGCCGATACCTTCAACGGCAATGCGGCG
GATCAAATTCCTCCTCCGTTACAGCCTCCGGGAACGGATATGACTGGAATTTCCTTCAGG
GATCAGCTCTGGATCAATTCATACCCTCTTGATCGTAACTACATCTTCGATTACTTCGCT
TTGTCTCCTTTCTACGATACAACTTGCAACAATGAGATTCTTCGGAGAAGATCCATTCAC
CCTCTCGATCTCTCTCACCTCTCGTAAGCTCCTCCTCACTGCTCTACAGATAGGGTTATG
TTGAAACTCATAAAGGTTTCAACTTTAGGTCGAATTAGGGTTTGGTCTCACCTGGGATAC
TGTTAGAGAAATGGAGATAGATTTAGGTGGAGAATTTAGTGACAATGTTTGATTGAAACC
ATTGTGATTATATTATGTAGGAAGATGACAGGTCTAGAATATATGTTAACTGATGCT

Last Updated on 06.12.2018 16:11