Sequences from confirmation amplicon


Confirmation sequences for line 345C06 and hit At2g31210 are shown.
Line ID 345C06
Locus At2g31210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13297334
Primers used for PCR PH73 - 8474, show primer details
   
Sequence Name 13-K017550-022-345-C06C-PH73
GenBank Accession LN491826 [GenBank]
Sequencing primer gene specific primer, PH73: 5'-CAGAAACTCAGAACCAAAACATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K017550-022-345-C06C-PH73
ACAACCATTTGATCAACCAAATGATTCAAGAATCGAATTGGGGTGTTTCTGAAGACAACT
CTAATTTCTTCATGAACACTTCACATCCAAACACAACAACAACTCCAATCCCTGATCTTC
TCAGCCTCTTGCATTTGCCTAGATGCTCTATGTCATTACCAAGCTCTGATATAATGGCGG
GTTCTTGCTTCACGTATGACCCGCTCTTTCACTTAAACCTTCCTCCACAACCTCCATTGA
TCCCTTCTAATGACTACTCAGGGTACTTGCTTGGTATTGATACCAATACTACTACTCAAA
GAGATGAGTCTAATGTTGGAGATGAAAATAACAATGCTCAGTTTGATAGCGGAATCATCG
AGTTTAGCAAAGAGATTAGGCGTAAAGGAAGAGGGAAGCGAAAGAACAAACCTTTTACTA
CAGAACGTGAGCGAAGATGTCACTTGAAGATGTCAACAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGC
   
Sequence Name 14-K017550-022-345-C06C-8409
GenBank Accession LN492289 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K017550-022-345-C06C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTTGACATCT
TCAAGTGACATCTTCGCTCACGTTCTGTAGTAAAAGGTTTGTTCTTTCGCTTCCCTCTTC
CTTTACGCCTAATCTCTTTGCTAAACTCGATGATTCCGCTATCAAACTGAGCATTGTTAT
TTTCATCTCCAACATTAGACTCATCTCTTTGAGTAGTAGTATTGGTATCAATACCAAGCA
AGTACCCTGAGTAGTCATTAGAAGGGATCAATGGAGGTTGTGGAGGAAGGTTTAAGTGAA
AGAGCGGGTCATACGTGAAGCAAGAACCCGCCATTATATCAGAGCTTGGTAATGACATAA
AGCATCTAAGCAAAT

Last Updated on 06.12.2018 16:11