Sequences from confirmation amplicon


Confirmation sequences for line 347D02 and hit T1G11 are shown.
Line ID 347D02
Locus T1G11 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1278796
Primers used for PCR ZZ64 - 8474, show primer details
   
Sequence Name 11-K022404-022-347-D02C-8409
GenBank Accession LN490923 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K022404-022-347-D02C-8409
AGATTTCCCGGACATGAAGCCATTTGATTACATTTATAATCAAATACAATAAAAAAAGTG
ATACTTATATGTTGCTTACATTGATCGATTACACTGAAGAGACATGACAACGCATTTTGC
TTCACGATCATGTATATACATAAACATGAAAGGTCCATTGGGACTTCGGAGTTTCTCTTT
TGCTCCTCTTTGGTCTAGTTTTAGAGAAATACAAGAATTTTAGGCATACTAGTTATGTAA
CTGTTATACGATTTTAATGAGCTACACTTTATATAGTATTTTTTTTTGCTAAAGAAAACT
TTATATACATTTTATTCACATTTTGCGACGATTATAACATAGACAGAGTTTATGGAGAAA
AATGTATAGAAAAAGAGTTAAGTTGAAAAACATAGAAATTACGCAGGGGAAATTTTAAAG
ACAAAATATAACTGTTTATGTTACTCTTTTTTTGGTATCAGATTATATTACTTTTGTTGG
TAGGTGTAATATTTAATAAGCTAATAATTATTATTTTTCTTAGAAAAGTAAATAAATGTG
TATAGACGGCTGTGGAATTTGAGGCAACGTGGTTAAACCTTCCATCAGGTGTGGCTACAC
ATCCCACAGACTTC
   
Sequence Name 12-K022404-022-347-D02C-ZZ64
GenBank Accession LN491384 [GenBank]
Sequencing primer gene specific primer, ZZ64: 5'-TAGTCTGTGGATTGAGGAAGTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K022404-022-347-D02C-zz64
ATGTGTAGCCACACCTGATGGAAGGTTTAACCACGTTGCCTCAAATTCCACAGCCGTCTA
TACACATTTATTTACTTTTCTAAGAAAAATAATAATTATTAGCTTATTAAATATTACACC
TACCAACAAAAGTAATATAATCTGATACCAAAAAAAGAGTAACATAAACAGTTATATTTT
GTCTTTAAAATTTCCCCTGCGTAATTTCTATGTTTTTCAACTTAACTCTTTTTCTATACA
TTTTTCTCCATAAACTCTGTCTATGTTATAATCGTCGCAAAATGTGAATAAAATGTATAT
AAAGTTTTCTTTAGCAAAAAAAAATACTATATAAAGTGTAGCTCATTAAAATCGTATAAC
AGTTACATAACTAGTATGCCTAAAATTCTTGTATTTCTCTAAAACTAGACCAAAGAGGAG
CAAAAGAGAAACTCCGAAGTCCCAATGGACCTTTCATGTTTATGTATATACATGATCGTG
AAGCANAATGCGTTGTCATGTCTCTTCAGTGTAATCGATCAATGTAAGCAACATATAAGT
ATCACTTTTTTTATTGTATTTGATTATAAATGTAATCAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATG

Last Updated on 06.12.2018 16:11