Sequences from confirmation amplicon


Confirmation sequences for line 349G01 and hit At1g64670 are shown.
Line ID 349G01
Locus At1g64670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24032486
Primers used for PCR AY20 - 8474, show primer details
   
Sequence Name 59-K018321-022-349-G01C-AY20
GenBank Accession LN509856 [GenBank]
Sequencing primer gene specific primer, AY20: 5'-AGTCTGAACTGAGAAATCACCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K018321-022-349-G01C-AY20
ATCTCTATCGATCTTTTATTGTATACAATGAATCGTTCGGCTTTGGACTTTTCCCATAAC
CCAACAGGTCCACTGCGAGAAACCTGTAATTCGATTTGGCTGAGTCTGAGAAGTTTGGGA
ACAGAGTCTCCGTCCAAAACGTGGATGATGATAAGAACCCATGAATGAACACTACGTTCT
CTTGTGCCTTGTTGTCTGCACATTCAATATTTAATATTTAGTTATGTCTTTAACCTCTCA
AAAATTAGTTTATATATTTCTTACAAAAATTGTTTCTGACCTAGTGTAGCACAAGCAATA
TTTGTTTTTTTGTTTTTTGAACAAGCAATATAATGTAAATAATTTATGATGTAATATGGA
TAAACTACTAAGAGATTTTTTCACTCAGCTTCTGAGTCTAAGTCTTTTCACTCCACATTA
GGTCTGCATTGTCTCATAAGTTCTTCACATTGTTTCTGAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCT
   
Sequence Name 63-K018321-022-349-G01C-8409
GenBank Accession LN510877 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K018321-022-349-G01C-8409
TAGATTTCCTCGGACATGAAGCCATTTACAATTCAGAAACAATGTGAAGAACTTATGAGA
CAATGCAGACCTAATGTGGAGTGAAAAGACTTAGACTCAGAAGCTGAGTGAAAAAATCTC
TTAGTAGTTTATCCATATTACATCATAAATTATTTACATTATATTGCTTGTTCAAAAAAC
AAAAAAACAAATATTGCTTGTGCTACACTAGGTCAGAAACAATTTTTGTAAGAAATATAT
AAACTAATTTTTGAGAGGTTAAAGACATAACTAAATATTAAATATTGAATGTGCAGACAA
CAAGGCACAAGAGAACGTAGTGTTCATTCATGGGTTCTTATCATCATCCACGTTTTGGAC
GGAGACTCTGTTCCCAAACTTCTCAGACTCAGCCAAATCGAATTACAGGTTTCTCGCAGT
GGACCTGTTGGGTTATGGGAAAAGTCCAAAGCCGAACGATTCATTGTATACATTAAAAGA
GCATTTAGAGATGATAGAGAGATCGGTGATTT

Last Updated on 06.12.2018 16:11