Sequences from confirmation amplicon


Confirmation sequences for line 351G01 and hit At3g12100 are shown.
Line ID 351G01
Locus At3g12100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3855399
Primers used for PCR RE31 - 8474, show primer details
   
Sequence Name 11-K025445-022-351-G01C-8409
GenBank Accession LN490949 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K025445-022-351-G01C-8409
GTAGATTTCCCGGACATGAAGCCATTTTTAAATATATCCGGCACTGAAAAGACAGCAGCA
AAGAAAACTTCATGTTGACTGTATATAAGTTTGATGCAAAAGGAAGCATTTTCTTTTCTA
TATAGCTTTCTCCGATTCTGCTTGAAATTTATAAAGGCAGAGAGGATTAACACCTGCGGA
TGGAATCTGATATGACGTGCAAGCAAACGGAGTGATAATTCATATCTTCTGCTTTTCTGT
AGGCTGCAGCATATTGCATATTATGAATATAAGAAAAAAATTCAAGATAACATGTGGTTT
TTAGATATGTAAAGAAGGGACAGATAATTTCAAGAAGCAACGAATAGTCGTTTGACAGAT
CAGTTACAATTATGCTGACTCTCTCTTTCAACTAATCTTCGTACTGATTCTATCTTAACC
AAACACCCTTACTTCTT
   
Sequence Name 12-K025445-022-351-G01C-RE31
GenBank Accession LN491408 [GenBank]
Sequencing primer gene specific primer, RE31: 5'-TTTAAAGCCAAAGAAGTAAGGGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K025445-022-351-G01C-RE31
TTTGGTTAAGATAGAATCAGTACGAAGATTAGTTGAAAGAGAGAGTCAGCATAATTGTAA
CTGATCTGTCAAACGACTATTCGTTGCTTCTTGAAATTATCTGTCCCTTCTTTACATATC
TAAAAACCACATGTTATCTTGAATTTTTTTCTTATATTCATAATATGCAATATGCTGCAG
CCTACAGAAAAGCAGAAGATATGAATTATCACTCCGTTTGCTTGCACGTCATATCAGATT
CCATCCGCAGGTGTTAATCCTCTCTGCCTTTATAAATTTCAAGCAGAATCGGAGAAAGCT
ATATAGAAAAGAAAATGCTTCCTTTTGCATCAAACTTATATACAGTCAACATGAAGTTTT
CTTTGCTGCTGTCTTTTCAGTGCCGGATATATTTAAAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11