Sequences from confirmation amplicon


Confirmation sequences for line 355B01 and hit At4g01100 are shown.
Line ID 355B01
Locus At4g01100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:479505
Primers used for PCR GL14 - 8474, show primer details
   
Sequence Name 19-K024724-022-355-B01C-8409
GenBank Accession LN494597 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K024724-022-355-B01C-8409
AAATTTCCCGGAACATGAAGCCATTTACAATTGAATATATCCTTACAGAAAGAAACACAA
ACAACACTTCACTTTCTTGAGTTCATCAAATAAGAAGATCAAGAAACGGAGTTTTGTTAT
GAAACAAACCTTCACTGAATTGGGGACCAAACCCTTGTACAATGCTCCAAAGCCTTCATG
ACGAACTGTTTTTCTAAATGCATCGACCATACCAGTGTATTCAAGTGAAGCTGTGCTTCT
CCCTTCGCCTGTTACAATAGCGGAAGCATCTTTCCACCCGACCATTTGCATTCTCCTACG
AATCACATCCAGTGGATAAGCAATTGTTTGACCAACTGTTCCAGCTATTGCACCACAAGT
GAGCCTCGTTACAACCGTCAGCTCATTGTTTTCTACTAGACCGTATGGGTTCTCTT
   
Sequence Name 20-K024724-022-355-B01C-GL14
GenBank Accession LN495051 [GenBank]
Sequencing primer gene specific primer, GL14: 5'-GACTGGCTAGTTAAAGAGAACCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K024724-022-355-B01C-GL14
GGTCTAGTAGAAAACAATGAGCTGACGGTTGTAACGAGGCTCACTTGTGGTGCAATAGCT
GGAACAGTTGGTCAAACAATTGCTTATCCACTGGATGTGATTCGTAGGAGAATGCAAATG
GTCGGGTGGAAAGATGCTTCCGCTATTGTAACAGGCGAAGGGAGAAGCACAGCTTCACTT
GAATACACTGGTATGGTCGATGCATTTAGAAAAACAGTTCGTCATGAAGGCTTTGGAGCA
TTGTACAAGGGTTTGGTCCCCAATTCAGTGAAGGTTTGTTTCATAACAAAACTCCGTTTC
TTGATCTTCTTATTTGATGAACTCAAGAAAGTGAAGTGTTGTTTGTGTTTCTTCTGTAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GAC

Last Updated on 06.12.2018 16:11