Sequences from confirmation amplicon


Confirmation sequences for line 355H08 and hit At1g06290 are shown.
Line ID 355H08
Locus At1g06290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1922934
Primers used for PCR 06sw - 8474, show primer details
   
Sequence Name 14-K116707-0022-355-H08C-06sw
GenBank Accession LN492676 [GenBank]
Sequencing primer gene specific primer, 06sw: 5'-AATGTGGAAGATCACCATGACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K116707-0022-355-H08C-06sw
TACTTCCATGGCCTAGCTCAGTCATAGCAAAACAGCCCTTGACAACATAATCTTCGGTGT
TCTTCAGCCACTTTTCATGGTGACGCTTTGTTCCAAAAAACTTTACAGCATTACCCCTGG
GTATCACAGAAAAACACTTATAAAGCCTAACACTGTTTTCTGTCTCCCTAGGACGCTAAA
TAACTTCAAAATTCTATAACCACATTTCATTGACCAAAGCACAAATTAATCTTTCTATTT
GAACTTTTCAATCTCAGATTAGGATTAATACCAAGCAGAGCACATCTCCACAATGAAAAA
AAATCATAGCTTGACGTAAATTAATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 01-K116706-0022-355-H08C-8409
GenBank Accession LN486740 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116706-0022-355-H08C-8409
AATTAATTTACGTTCAAGCTATGATTTTTTTTCATTGTGGAGATGTGCTCTGCTTGGTAT
TAATCCTAATCTGAGATTGAAAAGTTCAAATAGAAAGATTAATTTGTGCTTTGGTCAATG
AAATGTGGTTATAGAATTTTGAAGTTATTTAGCGTCCTAGGGAGACAGAAAACAGTGTTA
GGCTTTATAAGTGTTTTTCTGTGATACCCAGGGGTAATGCTGTAAAGTTTTTTGGAACAA
AGCGTCACCATGAAAAGTGGCTGAAGAACACCGAAGATTATGTTGTCAAGGGCTGTTTTG
CTATGACTGAGCTAGGCCATGGAAGTAATGTAGGCATATTTTCTTGATTGCCATTTTTTT
TCTCCATATGTCATGGTGATTTTTCCACAT

Last Updated on 06.12.2018 16:11