Sequences from confirmation amplicon


Confirmation sequences for line 358H06 and hit At4g03290 are shown.
Line ID 358H06
Locus At4g03290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1443361
Primers used for PCR GE26 - 8474, show primer details
   
Sequence Name 86-K033715-022-358-H06C-8409
GenBank Accession LN514609 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K033715-022-358-H06C-8409
GCCATTTACATTGACATGAAGTAATGTCAGTGTTGACCTTCTTAACTGGGAATCCAGTCA
TGGATTTGAGACCGCAAGAGAAAATGGTTAAGCAAATAACCAAAATTAAAAACAAGAGAA
GGTAAAAAAAGAAGAAAAAAAGTTATTATCAGCTCAAAGAGCTAAAAAAGCGACCCTTTT
TCATCATTTGTCTAAACTCCATGTAATTAACCCTACCATCACCATCAACATCCACTTGCA
TTATCATCTTCCTACATTCCTCCAAGGTCTTACCTTGCTTGAGTCCCAAGGAAGATAAAA
CCGCTTTCAATTCATCCACCGTTATAAACCCATCTCCGTTCCGATCAAATACGTTGAACG
CTTCCTTCATATCTTCTTCTCCTACCTCGTCCTCATCTTCTACCATTATCGTCTTGTAGA
GCTCTCCAAACTCTTCAATGTCAACACAACCGTCACCATTAACATCGATCTTTTGGATAA
TTTGTGTTAGTTCGTCTTCGGGTATTATGATACCGAGGTTCTTGAATGACTCGTTCAGCT
CCTTCTAGNTGATC
   
Sequence Name 06-K033715-022-358-H06C-GE26
GenBank Accession LN488702 [GenBank]
Sequencing primer gene specific primer, GE26: 5'-AAGATCACTACGAAGGAGCTGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K033715-022-358-H06C-GE26
TACCCGAAGACGAACTAACACAAATTATCCAAAGATCGATGTTAATGGTGACGGTTGTGT
TGACATTGAAGAGTTTGGAGAGCTCTACAAGACGATAATGGTAGAAGATGAGGACGAGGT
AGGAGAAGAAGATATGAAGGAAGCGTTCAACGTATTTGATCGGAACGGAGATGGGTTTAT
AACGGTGGATGAATTGAAAGCGGTTTTATCTTCCTTGGGACTCAAGCAAGGTAAGACCTT
GGAGGAATGTAGGAAGATGATAATGCAAGTGGATGTTGATGGTGATGGTAGGGTTAATTA
CATGGAGTTTAGACAAATGATGAAAAAGGGTCGCTTTTTTAGCTCTTTGAGCTGATAATA
ACTTTTTTTCTTCTTTTTTTACCTTCTCTTGTTTTTAATTTTGGTTATTTGCTTAACCAT
TTTCTCTTGCGGTCTCAAATCCATGACTGGATTCCCAGTTAAGAAGGTCAACACTGACAT
TACTTCATGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11