Sequences from confirmation amplicon


Confirmation sequences for line 358H08 and hit At2g41040 are shown.
Line ID 358H08
Locus At2g41040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17121985
Primers used for PCR CK75 - 8474, show primer details
   
Sequence Name 03-K026766-022-358-H08C-8409
GenBank Accession LN487332 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K026766-022-358-H08C-8409
TCCCGGACATGAAGCCATGTAGATTGCTTGTCTGCAGCAGATTCACAAAAATGCTTTAGA
GAAATAAGAAAAAGGGCAAAACTTTGAAATCATTTTAAAATCTGAGGAAGTAAGTAAAGA
ACTTACAGGTTGATACCTGAAGGTCCTTTCCTCATAAGTGGTTCGTAGCAAACAGGACAC
GCGAACACTTGAGTTTCCTCTATTTCGATCTTTGGAGTTTCATTTTTATTTATGTCCTG
   
Sequence Name 04-K026766-022-358-H08C-CK75
GenBank Accession LN487771 [GenBank]
Sequencing primer gene specific primer, CK75: 5'-ACCTTTCTTACCTAACCGCTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K026766-022-358-H08C-CK75
ACAATCTCCTCTTCTCCGATTCTCTGCTTCTACCTCTGTTCGTTCTCGTTTTCCATCCGC
AGCCATAAGCGCCGTTGCTCCCAAAACGGTGCGTTCTGCTTTTTTGTTAATTCACTTGGA
TTGATTTACAAGCTTTGTATATGTATGTAAACCTTGTACAATGGTATTTTCAGGACATAA
ATAAAAATGAAACTCCCAAGATCGAAATAGAGGAAACTCCAGTGGTCCCGTGTCCTGTTT
GCCACCAACCACTTATGAAGAAAGGACCTTCAGGTATCAACCTGTAAGTTCTTTACTTAC
TTCCTCAAATTGTAAAATGATTTCAAAGTTTTGCCCTTTTTCTTATTTCTCTAAAGCATT
TTTGTGAATCTGCTGCAAACAAACAATCTACATGGCTTCATGTCCGGGAAATCTACATGG
ATCAACAAT

Last Updated on 06.12.2018 16:11