Sequences from confirmation amplicon


Confirmation sequences for line 364C05 and hit At2g26330 are shown.
Line ID 364C05
Locus At2g26330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11210433
Primers used for PCR SD05 - 8474, show primer details
   
Sequence Name 13-K023017-022-364-C05C-8409
GenBank Accession LN491854 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K023017-022-364-C05C-8409
TAGATTTCCCGGACATGAAGCAATTTGTTTATCCCTTCCAAGCTGTTAAGAAATTGTTTT
TGTAGAATGAAACTAAAACTCTGTATACACAATAATGAGGTCACTATAGTGTGATCCAGG
AACATGTATTGGGTTGGTGATCTATCTAATGTTGTGTTTCTTAAAATTGCTTGCAGGAGA
CTGGAAAATAATAACCTGACTGGTAATGTTGGTTCATTAGCCAACTGTCTCAGTCTCACT
GTATTGTAAGTAGGCACCTTTGGTTCTGAAACATTTTTTGTCCCTCTTTGTGCATCTTTT
GCTAAGAATATAACCCTGCAATCTTCACTAACTCTTATAGGAATGTATCTCATAACAACC
TCGTAGGTGATATCCCTAAGAACAATAACTTCTCAAGATTTTCACCAGACAGGTATGGTA
ATTTAGCAGGTTTTGGTATTGTGCATTTTGTTTTGTTTGCTAATATCTATGTTTATGTTT
TTGGATAAAGCTTCATTGGCAATCCTGGTCTTTGCGGTAGTTGGCTAAACTCACCGTGTC
ATGATTCTCGTCGAACTGTACGAGGTGATTACATTCTTCTAAAAGCTTCCATTCACAAAA
CCTAAGATAATTAAAGCTCATGTTTCTAT
   
Sequence Name 14-K023017-022-364-C05C-SD05
GenBank Accession LN492322 [GenBank]
Sequencing primer gene specific primer, SD05: 5'-ACTGCAGACAAAACATGGATAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K023017-022-364-C05C-SD05
TGAGCTTTAATTATCTTAGGTTTTGTGAATGGAAGCTTTTAGAAGAATGTAATCACCTCG
TACAGTTCGACGAGAATCATGACACGGTGAGTTTAGCCAACTACCGCAAAGACCAGGATT
GCCAATGAAGCTTTATCCAAAAACATAAACATAGATATTAGCAAACAAAACAAAATGCAC
AATACCAAAACCTGCTAAATTACCATACCTGTCTGGTGAAAATCTTGAGAAGTTATTGTT
CTTAGGGATATCACCTACGAGGTTGTTATGAGATACATTCCTATAAGAGTTAGTGAAGAT
TGCAGGGTTATATTCTTAGCAAAAGATGCACAAAGAGGGACAAAAAATGTTTCAGAACCA
AAGGTGCCTACTTACAATACAGTGAGACTGAGACAGTTGGCTAATGAACCAACATTACCA
GTCAGGTTATTATTTTCCAGTCTCCTGCAAGCAATTTTAAGAAACACAACATTAGATAGA
TCACCAACCCAATACATGTTCCTGGATCACACTATAGTGACCTCATTATTGTGTATACAG
AGTTTTAGTTTCATTCTACAAAAACAATTTCTTAACAGCTTGGAAGGGATAAACAAATTG
CTTCATGTCCGGGAAATCTACATGGATCANCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAA

Last Updated on 06.12.2018 16:11