Sequences from confirmation amplicon


Confirmation sequences for line 365G02 and hit At5g10740 are shown.
Line ID 365G02
Locus At5g10740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3395164
Primers used for PCR PC82 - 8474, show primer details
   
Sequence Name 13-K103845-0022-365-G02C-PC82
GenBank Accession LN492010 [GenBank]
Sequencing primer gene specific primer, PC82: 5'-TATTGGTGGTGCCATACAAAGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K103845-0022-365-G02C-PC82
ATATCCACCAAAAACAAAAAAAGATAAGTCATAACGGATCTCATAGAAATTTACCTTTTC
CGCCTCTGCTGATAACAGCTCTAGAATCACCAACATTTGCAACAACTAAACGGTCACCAA
CAAGAATAGCTGTAGACGCAGTAGAACCAGCGTCTCTATTGTGACTATTTTCTGACTTGA
GAAGTTCGGAGTCTGTATGGTTATAAGCATCAGCTGGAAAACATTGAAGAAAATGGAATG
TAAATTAGAGTCCTGCTGCATCTACNACAAATTGTTTTAAAAAAGCTTTGCTCATGATTT
TTTTACAGTACTTATAGCTGATTTGGTGTCAGAGATAAACTTTGGGTGGGTGATCAGATT
ACTGAAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 29-K103846-0022-365-G02C-8409
GenBank Accession LN499255 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K103846-0022-365-G02C-8409
CCATTTTTAGTAATCTGATCACCCACCCAAAGTTTATCTCTGACACCAAATCAGCTATAA
GTACTGTAAAAAAATCATGAGCAAAGCTTTTTAAAACAATTTGTTGTAGATGCAGCAGGA
CTCTAATTTACATTCCATTTTCTTCAATGTTTTCCAGCTGATGCTTATAACCATACAGAC
TCCGAACTTCTCAAGTCAGAAAATAGTCACAATAGAGACGCTGGTTCTACTGCGTCTACA
GCTATTCTTGTTGGTGACCGTTTAGTTGTTGCAAATGTTGGTGATTCTAGAGCTGTTATC
AGCAGAGGCGGAAAAGGTAAATTTCTATGAGATCCGTTATGACTTATCTTTTTTTGTTTT
TGGTGGATATCATTGTAATGTTAAGTAAGCAATATCTTTGTATGGCACCACCAATA
Primers used for PCR PC83 - 8474, show primer details
   
Sequence Name 40-K104195-0022-365-G02C-PC83
GenBank Accession LN503694 [GenBank]
Sequencing primer gene specific primer, PC83: 5'-TGTTCGATTCGATTTATCTCACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K104195-0022-365-G02C-PC83
GATTTTTGGTGTGTTAATGTTTATTGTGCTTTGTACATGATATGATTCTATTGCAGCCAG
AATGGAAAATTTAGCTATGGATATGCAAGCTCCGCTGGCAAGAGATCATCTATGGAAGAC
TTTTTTGAAACTAGGATCGACGGTATCAATGGAGAAATAGTTGGTCTATTTGGAGTTTTT
GATGGTATCTTCCCTCTCTGTTCTTAACAAGAACTTATACATTACTCTTGAGAAACTTAG
CCTGGTTTATGAATCCTGATAGTTCATTTGTGCCAGGAGTCATGACATTAAGATTGGTCT
TGGTGCTAATTAACTTCGTGTTCTCGCTGTAACGTAGCTNATATCTTAGTATTGACATTA
GAAAACAAGAAATTAATAATGTTACTTACTGTTTTGCCAGGCCATGGTGGAGCCAGAGCA
GCTGAGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCA
   
Sequence Name 56-K104196-0022-365-G02C-8409
GenBank Accession LN509089 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K104196-0022-365-G02C-8409
GAGCCATTTAAATTGACTCAGCTGCTCTGGCTCCACCATGGCCTGGCAAAACAGTAAGTA
ACATTATTAATTTCTTGTTTTCTAATGTCAATACTAAGATATAAGCTACGTTACAGCGAG
AACACGAAGTTAATTAGCACCAAGACCAATCTTAATGTCATGACTCCTGGCACAAATGAA
CTATCAGGATTCATAAACCAGGCTAAGTTTCTCAAGAGTAATGTATAAGTTCTTGTTAAG
AACAGAGAGGGAAGATACCATCAAAAACTCCAAATAGACCAACTATTTCTCCATTGATAC
CGTCGATCCTAGTTTCAAAAAAGTCTTCCATAGATGATCTCTTGCCAGCGGAGCTTGCAT
ATCCATAGCTAAATTTTCCATTCTGGCTGCAATAGAATCATATCATGTACAAAGCACAAT
AAACATTTAACACACCAAAAATCACACTTTATGATAGGAAAACAGAATGAACCAGTGAGA
TAAATCGAATCGAAC

Last Updated on 06.12.2018 16:11