Sequences from confirmation amplicon


Confirmation sequences for line 365G09 and hit At5g61780 are shown.
Line ID 365G09
Locus At5g61780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24822558
Primers used for PCR 37ny - 8474, show primer details
   
Sequence Name 02-K114626-0022-365-G09C-37ny
GenBank Accession LN487138 [GenBank]
Sequencing primer gene specific primer, 37ny: 5'-CCCACAGAGATAAAAACCCAAATA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114626-0022-365-G09C-37ny
CATAGATAGAATTATACCACTGGTTAGAAACTTCGACAGCCCAACAAGTTTTTCCTCAGT
ACTGAACTCTAGACGTGTTTTGATAAACCAAATTTAGAGAAACAGACTAAGAGACACCAA
CCTTGCTCCAACGACCAAATCCTTCCTGCTGAGCCTGCTCTTCAAGCTGTTCCAGCTCCG
CAATGTAAGGACTAACCTTATCCTGATTCTGCTGACCTGGCCGTCTGACCTGTGCATATA
AAGTACAAGCATTCCTCCACCGTATGAGAAAGCTGATGCGAGCATCATACCTTAGACAAG
GCTATCTATGAAATAAAGCGGTAATCACCTTTGCCCAGCCATTTTGAAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 02-K114625-0022-365-G09C-8409
GenBank Accession LN487137 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K114625-0022-365-G09C-8409
TTTCAAATGGCTGGGCAAGGTGATTACCGCTTTATTTCATAGATAGCCTTGTCTAAGGTA
TGATGCTCGCATCAGCTTTCTCATACGGTGGAGGAATGCTTGTACTTTATATGCACAGGT
CAGACGGCCAGGTCAGCAGAATCAGGATAAGGTTAGTCCTTACATTGCGGAGCTGGAACA
GCTTGAAGAGCAGGCTCAGCAGGAAGGATTTGGTCGTTGGAGCAAGGTTGGTGTCTCTTA
GTCTGTTTCTCTAAATTTGGTTTATCAAAACACGTCTAGAGTTCAGTACTGAGGAAAAAC
TTGTTGGGCTGTCGAAGTTTCTAAACCAGTGGTATAATTCTATCTATTGTCTACTCAAAA
TATGGGGAACAAACTTGAAGTTATTTGGGTTTTTACTTCTGTGGG

Last Updated on 06.12.2018 16:11