Sequences from confirmation amplicon


Confirmation sequences for line 366A12 and hit At3g47070 are shown.
Line ID 366A12
Locus At3g47070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17337612
Primers used for PCR BD44 - 8474, show primer details
   
Sequence Name 54-K114362-0022-366-A12C-BD44
GenBank Accession LN508555 [GenBank]
Sequencing primer gene specific primer, BD44: 5'-GTCAGGGACAAGATCACACACTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K114362-0022-366-A12C-BD44
TTCATTTGACAATAAATTATAAATCTGAAATTGAAGACTTTGACTCTCTGTGCAAATTTC
CGTTTAAGAACTTGAGAAGAATATACATAAATTGTGCCAAGATATATCTAAAAACAATTA
AACCAAAAAAAATGTATCCAAGTGATTCAAAGAAAAATGTGTGAAGGGAAAAAAGAAAGA
GGATAAGACACTTGTGGAGAGCTTGTGCCAAAAAAATCCAACGGCTCAGATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 24-K114361-0022-366-A12C-8409
GenBank Accession LN497092 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114361-0022-366-A12C-8409
ATCTGAGCCGTTGGATTTTTTTGGCACAAGCTCTCCACAAGTGTCTTATCCTCTTTCTTT
TTTCCCTTCACACATTTTTCTTTGAATCACTTGGATACATTTTTTTTGGTTTAATTGTTT
TTAGATATATCTTGGCACAATTTATGTATATTCTTCTCAAGTTCTTAAACGGAAATTTGC
ACAGAGAGTCAAAGTCTTCAATTTCAGATTTATAATTTATTTGTCAAATGAAATGTATGA
ATGCGGAAGAGACACGACTTGATTTTTTTTAAGTGTGTGATCTGNTCCCT

Last Updated on 06.12.2018 16:11