Sequences from confirmation amplicon


Confirmation sequences for line 367A12 and hit F6I1 are shown.
Line ID 367A12
Locus F6I1 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5828400
Primers used for PCR XD02 - 8474, show primer details
   
Sequence Name 69-K021646-022-367-A12C-8409
GenBank Accession LN512111 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K021646-022-367-A12C-8409
CATTTTTATTATTTCTTGGTAAAAAAAACATTACCATAATTAACCTCTAATAAGGGATTA
GGGACCAAGCAAATATGTAGTTGGAACATAAATGGGCCACACAAATATAAAAACTGAGCA
GAAGAAGCAAAAATCGCAACTCCTTTCTTTCTTGTCGGTAATTGATTTTAGATAAACAAA
TATACTTAAGTCTTACAATAAAAATCCTTTCCACCTAATTTAAATCACTGAGCTTTACAA
AAGTTAGTATCAGTTAATACTTAGACTTAGCAAATCTATCTGTACAATAAACCTCAATAA
CAGTCGATGCTTCTCTACTTTGTTGAGTTGAGTCTGTTGATTAGATTCAGATGCCTCTTA
TAATCCTGAAAAATGCAACAAAAACCAAAATAAATAAAATAAGCAATTTAAGATCTTCAA
TTTGATCTCTCTTTGCTTCAACAAAAACTAAATTTTTTCACTTCCTCACTCACCTTCCAA
GCCGAGATAGCTCAGGTTCCGCCAAAAGCATATCTGGCAACTGTT
   
Sequence Name 70-K021646-022-367-A12C-XD02
GenBank Accession LN512295 [GenBank]
Sequencing primer gene specific primer, XD02: 5'-GGTATACAGATGCAAAACAGTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K021646-022-367-A12C-XD02
CTCGGCTTGGAAGGTGAGTGAGGAAGTGAAAAAATTTAGTTTTTGTTGAAGCAAAGAGAG
ATCAAATTGAAGATCTTAAATTGCTTATTTTATTTATTTTGGTTTTTGTTGCATTTTTCA
GGATTATAAGAGGCATCTGAATCTAATCAACAGACTCAACTCAACAAAGTAGAGAAGCAT
CGACTGTTATTGAGGTTTATTGTACAGATAGATTTGCTAAGTCTAAGTATTAACTGATAC
TAACTTTTGTAAAGCTCAGTGATTTAAATTAGGTGGAAAGGATTTTTATTGTAAGACTTA
AGTATATTTGTTTATCTAAAATCAATTACCGACAAGAAAGAAAGGAGTTGCGATTTTTGC
TTCTTCTGCTCAGTTTTTATATTTGTGTGGCCCATTTATGTTCCAACTACATATTTGCTT
GGTCCCTAATCCCTTATTAGAGGTTAATTATGGTAATGTTTTTTTTACCAAGAAATAAAT
AAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11