Sequences from confirmation amplicon


Confirmation sequences for line 368G05 and hit At3g63280 are shown.
Line ID 368G05
Locus At3g63280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23381299
Primers used for PCR GD90 - 8474, show primer details
   
Sequence Name 75-K033721-022-368-G05C-8409
GenBank Accession LN513180 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K033721-022-368-G05C-8409
GCCATTTACAATTGAGCCATTTACTCGGCTCCAAGGGTTTAAGCAGAACCCCGAGCTCAT
CGTACCGTTCCTGCTGCAAAAGCTTTGCACTGAACTCCAGCAGTCCCTCCAGAGCCTCCG
CACGCTGCTGATACGATGACGTGTCGAACCTTCTCTGTTTTACCTCCGAAACTGACCTTT
TCTGTACAGTACACTTGTCCTTTGTGATCGAACCGTTGGAATTGTCCTCACCATGAGGCG
GACAAGGAGGAGAAGAAAAAGAGTGCCTAGCTACTTCTCTTCTTGACGCTGTCTCTCTGT
TTTGGTTTTGGAAGATATCTTCAGGGAATTCAGCGATCTTGTCGAATCTTGGAGTGTTTA
CAGAAACATCCGGTGAATTTAGGGAACAAAGAGCTG
   
Sequence Name 37-K033721-022-368-G05C-GD90
GenBank Accession LN502522 [GenBank]
Sequencing primer gene specific primer, GD90: 5'-CAGCTCTTTGTTCCCTAAATTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K033721-022-368-G05C-CD90
TCCAGATCGACAGATCGCTGAATTCCCTGAAGATATCTTCCAAAACCAAAACAGAGAGAC
AGCGTCAAGAAGAGAAGTAGCTAGGCACTCTTTTTCTTCTCCTCCTTGTCCGCCTCATGG
TGAGGACAATTCCAACGGTTCGATCACAAAGGACAAGTGTACTGTACAGAAAAGGTCAGT
TTCGGAGGTAAAACAGAGAAGGTTCGACACGTCATCGTATCAGCAGCGTGCGGAGGCTCT
GGAGGGACTGCTGGAGTTCAGTGCAAAGCTTTTGCAGCAGGAACGGTACGATGAGCTCGG
GGTTCTGCTTAAACCCTTTGGAGCCGAGTAAATGGCTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAA
GTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAAC
AAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCNAAATGG

Last Updated on 06.12.2018 16:11