Sequences from confirmation amplicon


Confirmation sequences for line 368H02 and hit At1g17880 are shown.
Line ID 368H02
Locus At1g17880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6152583
Primers used for PCR MJ37 - 8474, show primer details
   
Sequence Name 57-K030444-022-368-H02C-8409
GenBank Accession LN509313 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K030444-022-368-H02C-8409
CAGCAGCAGCTGGTGCTTTCTCTTCAGCAGCAGTTTCGAATGTCTCTCCCCACTCATCTG
GGACATCGTCATCATCCTCTTCTTGTATGGCTGTTGAGGCGGCATTACCTTCACCAGAAG
CCTGTTTCTGGAACTGTTCTGCTAGCTTCTTCAGGTTGTCCATGTTGTCTGGTCCTGAAA
GAGATCAAAGTAACATAAAGTTAGAGACCTTAGGATCGATGAAGAACATTTAAGAGCTGT
ACAGTTACATGGTACACACCAAGTTGGCTGATGATCTGAGGAAGGATATCTTGCAATTCT
GCAGGAAGCAACACTTNTACATTA
   
Sequence Name 58-K030444-022-368-H02C-MJ37
GenBank Accession LN509603 [GenBank]
Sequencing primer gene specific primer, MJ37: 5'-TTAATGTAAACGTGTTGCTTCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K030444-022-368-H02C-MJ37
AAACAGTTCCAGAAAACAGGCTTTCTCGGTGAAGGTAATCGCCGCCTCAGCAACCATACA
AGAAGTGGATGATGACGATGTCCCAGAGCTTGTTGGAGAGACATTCGAAACTCGCTGCTG
AAGAGAAAGCACCAGCTGCTGCTGCTTCTTCTTAGAGAGAAAAGAGCGACCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11