Sequences from confirmation amplicon


Confirmation sequences for line 369A03 and hit At1g12980 are shown.
Line ID 369A03
Locus At1g12980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4430768
Primers used for PCR BW07 - 8474, show primer details
   
Sequence Name 61-K021645-022-369-A03C-8409
GenBank Accession LN510401 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K021645-022-369-A03C-8409
TCGGATGAGTCTTTCGATCTTCGGCAAGTACAGCCTAACTGAGTTCCGTACATCAACATT
TCTTGATACCCCCACTCGTTTGATCCCAACGATAAACCACCGTCTGCGACGGCTCTTACG
CCGTCGTCATTAGCTTGAAAATTCCCGTAGCGATCTAGTTTCGACGACAAAGTCTCCTTA
GTCTCTTGAACCATATTTTCAGAGTATATAGTGAGATTAGAGAAATAACCAGAGTTGTCA
TGATGGCTAGTCACCGGCGGTGGTGTTGGTGGAGAAGGAGGAACGCCGCGATTTTTCTTC
AAGAACTCTTGAACGACCTCTTGTAATAGACCAGTATCAGAAGATTCTTCCGGGAAAAAC
GACTCGTTGTTTTCGTTTTCGGAATAAGAAACGCATGCCGTTTTCGATGCTGAGTAAGAA
GATGATGAGCCGTTGAAAGAGTTATTATACGGAGTCGTTTTACGAGAGGAACACGAGGCG
TCGCGTAAGAAGATAGACTGTGTATTATAGATCCTCTGCGCTGCCGGTGCACCGTAAAAG
TTTTGGGTAGAGGAATCTAACGGTAGAGAAGGAAGA
   
Sequence Name 62-K021645-022-369-A03C-BW07
GenBank Accession LN510643 [GenBank]
Sequencing primer gene specific primer, BW07: 5'-CTCGTTGTCCTCTTCCTTCTCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K021645-022-369-A03C-BW07
GCACCGGCAGCGCAGAGGATCTATAATACACAGTCTATCTTCTTACGCGACGCCTCGTGT
TCCTCTCGTAAAACGACTCCGTATAATAACTCTTTCAACGGCTCATCATCTTCTTACTCA
GCATCGAAAACGGCATGCGTTTCTTATTCCGAAAACGAAAACAACGAGTCGTTTTTCCCG
GAAGAATCTTCTGATACTGGTCTATTACAAGAGGTCGTTCAAGAGTTCTTGAAGAAAAAT
CGCGGCGTTCCTCCTTCTCCACCAACACCACCGCCGGTGACTAGCCATCATGACAACTCT
GGTTATTTCTCTAATCTCACTATATACTCTGAAAATATGGTTCAAGAGACTAAGGAGACT
TTGTCGTCGAAACTAGATCGCTACGGGAATTTTCAAGCTAATGACGACGGCGTAAGAGCC
GTCGCAGACGGTGGTTTATCGTTGGGATCAAACGAGTGGGGGTATCAAGAAATGTTGATG
TACGGAACTCAGTTAGGCTGTACTTGCCGAAGATCGTAAATGACTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATNTCGACGTGTCT

Last Updated on 06.12.2018 16:11