Sequences from confirmation amplicon


Confirmation sequences for line 371A12 and hit At1g47230 are shown.
Line ID 371A12
Locus At1g47230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17306496
Primers used for PCR BE69 - 8474, show primer details
   
Sequence Name 05-K029488-022-371-A12C-8409
GenBank Accession LN488225 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K029488-022-371-A12C-8409
CATTTACAATTGAAGGGTAAAAAGGAGAAAACAGCCGCCAAATAAATGTGGAAATGAAAT
CTGCAAAAAAGCCCCAAATTTTGGATACTCAAAAGTCAAATCCCTAAAAATTTCGAAATC
GGAAAATTTACACAGAGAAGCGCCATGACCTAAAAATCTCAAAACTTCCCCAACAAAAAT
GGCGCTTTTACTCAACCCATTTCCTTTTACCTACCTTGTACATTACCCCTTTTCTCAAAT
CTCACTTTCCCACTGAAATAACCCCATGGCGGAGAATCAGAACTGTGCGAGGATGACGAG
AGCGGCGGCGAAACGAAAAGCGTCGTCCATGGCGTTAGACGAAAACCCAGTTAGTAAGAA
GAGAGTTGTTCTCGGAGAGCTTCCGAATATGTCCAATGTCGTTGCTGTACCCAATCAAGA
AAGAGAGACCCTTAAGGCTAAAACAAGTGTTAATACCTCGAAGAGGCAGATGAAGAAGGC
TTTGATGATTCCTGAAGCTAGCGTACTAATCGAATCGAGATCTGTTGATCCTCAGATGTG
TGAGCCTTTTGCTAGTGATATTTGTGCTTATCTCCGTGAAATGGAGGTAAAAAAAAAATC
ATCTTTAGTAGATTTCTTGATTCCATCATTTTTGATCTTAGTGATCTTATGGTATCAGTT
TATGTTATTTTGGAATTGGTACAGTGTTGGACTTGATATTGATTAATGGGTTTGGTTAAA
GTTGTGGATTTGATAAGTTTCTGAGATTTTG
   
Sequence Name 06-K029488-022-371-A12C-BE69
GenBank Accession LN488677 [GenBank]
Sequencing primer gene specific primer, BE69: 5'-CTGTGATTGGACAAAATCTCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K029488-022-371-A12C-BE69
CACTTTACCAAACCATTAATCAATATCAAGTCCAACACTGTACCAATTCCAAAATAACAT
AAACTGATACCATAAGATCACTAAGATCAAAAATGATGGAATCAAGAAATCTACTAAAGA
TGATTTTTTTTTTACCTCCATTTCACGGAGATAAGCACAAATATCACTAGCAAAAGGCTC
ACACATCTGAGGATCAACAGATCTCGATTCGATTAGTACGCTAGCTTCAGGAATCATCAA
AGCCTTCTTCATCTGCCTCTTCGAGGTATTAACACTTGTTTTAGCCTTAAGGGTCTCTCT
TTCTTGATTGGGTACAGCAACGACATTGGACATATTCGGAAGCTCTCCGAGAACAACTCT
CTTCTTACTAACTGGGTTTTCGTCTAACGCCATGGACGACGCTTTTCGTTTCGCCGCCGC
TCTCGTCATCCTCGCACAGTTCTGATTCTCCGCCATGGGGTTATTTCAGTGGGAAAGTGA
GATTTGAGAAAAGGGGTAATGTACAAGGTAGGTAAAAGGAAATGGGTTGAGTAAAAGCGC
CATTTTTGTTGGGGAAGTTTTGAGATTTTTAGGTCATGGCGCTTCTCTGTGTAAATTTTC
CGATTTCGAAATTTTTAGGGATTTGACTTTTGAGTATCCAAAATTTGGGGCTTTTTTGCA
GATTTCATTTCCACATTTATTTGGCGGCTGTTTTCTCCTTTTTACCCTTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA

Last Updated on 06.12.2018 16:11