Sequences from confirmation amplicon


Confirmation sequences for line 372E03 and hit At5g60740 are shown.
Line ID 372E03
Locus At5g60740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24426547
Primers used for PCR MD12 - 8474, show primer details
   
Sequence Name 45-K032417-022-372-E03C-8409
GenBank Accession LN505424 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K032417-022-372-E03C-8409
GAGCCTTTACAATTGAATAATCCAGGATCCCCGGTTGCTTTCTCTCCATAGTGTAGTTTA
TCCAGAGAGAAAGATCTTAATGCAGTAATCTTACACAAGAGAGCTGAAAAAAACAAAAAA
TTGTTGCCAGAATAGATGAAAGAAGCAAAGTTGTTGTCAAAATAGCTTGAAAGTTTCTAC
TTACAAACAGCAATAACTGTATATGTATATCCCATGGCACCAAACGTTTCATCGCTCACT
TTAGCAAGCGTTCCTAAGCAGATACCAGCAAGTAAAAGTATTAGGTAATCTACAGCTAGT
GTCCTTGCTTCTCGTAGTCTTTGTTTACCTAACCTGCAAAGCAAACACATCGAGGTTAAG
AAAAGGATTCTCTGTGAATCGATTGATTTTGCAAGTATTTGGAAGGGATCTACCTTCCTA
AGAAGTATCTGTATTGCTGATACACACCAGGAACTTCTCTTTCGGATAAGTCACCTGAAC
   
Sequence Name 45-K032417-022-372-E03C-MD12
GenBank Accession LN505425 [GenBank]
Sequencing primer gene specific primer, MD12: 5'-AGTTCAGGTGACTTATCCGAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K032417-022-372-E03C-MD12
CATACAGATCTTCTTAGGAGGTAGATCCCTTCCAATACTTGCAAAATCAATCGATTCACA
GAGAATCCTTTTCTTAACCTCGATGTGTTTGCTTTGCAGGTTAGGTAAACAAAGACTACG
AGAAGCAAGGACACTAGCTGTAGATTACCTAATACTTTTACTTGCTGGTATCTGCTTAGG
AACGCTTGCTAAAGTGAGCGATGAAACGTTTGGTGCCATGGGATATACATATACAGTTAT
TGCTGTTTGTAAGTAGAAACTTTCAAGCTATTTTGACAACAACTTTGCTTCTTTCATCTA
TTCTGGCAACAATTTTTTGTTTTTTTCAGCTCTCTTGTGTAAGATTACTGCATTAAGATC
TTTCTCTCTGGATAAACTACACTATTGGAGAGAAAGCAACCGGGGATCCTGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
TACATT
Primers used for PCR 11em - 8474, show primer details
   
Sequence Name 53-K035338-022-372-E03C-8409
GenBank Accession LN508072 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K035338-022-372-E03C-8409
TGAGCCATTTACAATTGAATATATCCTGGCTTACTTCCTAGCGAAAGACACAGTTGATCA
CTTCAACACCATCGTTAAGCCGCTTGTTTATCTATCCATGTTCTACTTCTTCAACAATCC
AAGATCAACAGTTACAGACAATTATGTTGTCCTCATCTGTCTTGTATACTGTGTGACTGG
TATAGCTTATACCCTGGCCATACTTTTCGAACCTGGTCCAGCTCAACTAGTAAGTGTCTC
ATCAATGCTGTTGATAGAACCAAAGACTCG
   
Sequence Name 53-K035338-0022-372-E03C-11em
GenBank Accession LN508071 [GenBank]
Sequencing primer gene specific primer, 11em: 5'-AGTCCGAGTCTTTGGTTCTATCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K035338-0022-372-E03C-11em
GTTGAGCTGGACCAGGTTCGAAAGTATGGCCAGGGTATAAGCTATACCAGTCACACAGTA
TACAAGACAGATGAGGACAACATAATTGTCTGTAACTGTTGATCTTGGATTGTTGAAGAA
GTAGAACATGGATAGATAAACAAGCGGCTTAACGATGGTGTTGAAGTGATCAACTGTGTC
TTTCGCTAGGAAGTAAGCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGGTCCAAATGGG

Last Updated on 06.12.2018 16:11