Sequences from confirmation amplicon


Confirmation sequences for line 372G04 and hit At3g01270 are shown.
Line ID 372G04
Locus At3g01270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:83147
Primers used for PCR FQ61 - 8474, show primer details
   
Sequence Name 45-K034066-022-372-G04C-FQ61
GenBank Accession LN505441 [GenBank]
Sequencing primer gene specific primer, FQ61: 5'-AATGACAACCCAAGTTAACCAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K034066-022-372-G04C-FQ61
TTTTTGGGGTTCTGTCTTTAGGTGATGTTGCTTGGTGCACAAAACAACAACATGGATGAC
AAGAAGATGCAAGTCACAGTGGCTTACAACCATTTCGGAAAAGGACTTGTGCAGAGGATG
CCTAGGGTCCGATGGGGTTTCGTCCACGTTGTCAACAATGACTACACTCATTGGGAGCTT
TACGCGATCGGTGGTAGCCAAGGTCCTACCATTCTCAGCCATGGAAACCGTTTCATCGCT
CCTCCTCACAAACAACACTACAGAGAGGTCCAAAATGATTTGAGTGTGCACATATATATA
TGGCATCAAATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATTCACTCCCAAATGG
   
Sequence Name 29-K034066-022-372-G04C-8409
GenBank Accession LN499191 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K034066-022-372-G04C-8409
AAGCCATTTACAATTGATTTGATGCCATATATATATGTGCACACTCAAATCATTTTGGAC
CTCTCTGTAGTGTTGTTTGTGAGGAGGAGCGATGAAACGGTTTCCATGGCTGAGAATGGT
AGGACCTTGGCTACCACCGATCGCGTAAAGCTCCCAATGAGTGTAGTCATTGTTGACAAC
GTGGACGAAACCCCATCGGACCCTAGGCATCCTCTGCACAAGTCCTTTTCCGAAATGGTT
GTAAGCCACTGTGACTTGCATCTTCTTGTCATCCATGTTGTTGTTTTGTGCACCAAGCAA
CATCACCTAAAGACAGAACCCAAAAACATAACAAGATCCTTTGGTTAACTTGGGTTGTCA

Last Updated on 06.12.2018 16:11