Sequences from confirmation amplicon


Confirmation sequences for line 373C08 and hit At5g43810 are shown.
Line ID 373C08
Locus At5g43810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17616251
Primers used for PCR JP12 - 8474, show primer details
   
Sequence Name 58-K026684-022-373-C08C-8409
GenBank Accession LN509589 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K026684-022-373-C08C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCGTTGGTATAACAGAGATT
GTTAGTCAGAGATTGAATACCATCTGCTGTGAAATTGTTCTCGTCCCAAAGAACATGGTA
ATGTGCAGGCCTGCTTGTTCCCTAGATCATACAACAAACACAAACAGTGTCATTTTATCT
GTATAGTTACGGAAAAGGTCATATTAACGGATATTTCGATAGTTGTTTCGTACCTGAATA
CCCGCATGGCTACAAAGGTAGAAGTCGAATTCAGTTGGATGACATATTTTAGTGTCAACT
ACAGTACCTGAAACAAAAGGTGGCAAAGAAGCAGTTAGTTAGTTAGTTAATTATTTCTGT
TATGATCTTCTTTCAACCGTTTTTAATAACTAACCTGGTAAGATATTTCCGCTTCGGTCA
GTACTGTTTTTGTCTCGGTGATTATTAGCAAACAAACGAGTGTGGTGACGCTTCTGT
   
Sequence Name 59-K026684-022-373-C08C-JP12
GenBank Accession LN509879 [GenBank]
Sequencing primer gene specific primer, JP12: 5'-TCATAGTTGTACAGAAGCGTCACC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K026684-022-373-C08C-JP12
ACACTCGTTTGTTTGCTAATAATCACCGAGACAAAAACAGTACTGACCGAAGCGGAAATA
TCTTACCAGGTTAGTTATTAAAAACGGTTGAAAGAAGATCATAACAGAAATAATTAACTA
ACTAACTAACTGCTTCTTTGCCACCTTTTGTTTCAGGTACTGTAGTTGACACTAAAATAT
GTCATCCAACTGAATTCGACTTCTACCTTTGTAGCCATGCGGGTATTCAGGTACGAAACA
ACTATCGAAATATCCGTTAATATGACCTTTTCCGTAACTATACAGATAAAATGACACTGT
TTGTGTTTGTTGTATGATCTAGGGAACAAGCAGGCCTGCACATTACCATGTTCTTTGGGA
CGAGAACAATTTCACAGCAGATGGTATTCAATCTCTGACTAACAATCTCTGTTATACCAA
CGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11