Sequences from confirmation amplicon


Confirmation sequences for line 373H03 and hit At5g62575 are shown.
Line ID 373H03
Locus At5g62575 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25118430
Primers used for PCR MJ29 - 8474, show primer details
   
Sequence Name 24-K029910-022-373-H03C-8409
GenBank Accession LN496877 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K029910-022-373-H03C-8409
TGCTATAATCTATGTCAAAGCTGTGATGAAAAAAGAAGCTCTGGCTGCAGGGAAAAGCTC
ATGAGTGTTCTTGTCTTTGTATGCCTTTTTGTTTTATCCTTTATACAGTGTCTCTTTCTA
ATAATCTCACAATAATGTTTGTTTACTCAATGAAAATGGCATCTAATAAAGTAGACTAAA
AGAAAAAGACATTGTCCAGGGAAACCAAACTCTGGTTTTTTCTGACGTTGTGTGGAATCT
CTGCTTTTTCTTCTTTTTAAATATTCCTTAGCTTTTCACTTATTTGTACTTTGCTTAAAG
ATACACTCTCCATTTATAGATAACTGCAACGATTAATGTCTTATCCATGGATTAGGTGAC
TGTATTTATGTGAAATTGTGAATGATCAATCACACTTAATGGTTTACACTGGACATGAAA
   
Sequence Name 24-K029910-022-373-H03C-MJ29
GenBank Accession LN496878 [GenBank]
Sequencing primer gene specific primer, MJ29: 5'-ATCCTTTCGAAACAATTTCATGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K029910-022-373-H03C-MJ29
ATCATTCACAATTTCACATAAATACAGTCACCTAATCCATGGATAAGACATTAATCGTTG
CAGTTATCTATAAATGGAGAGTGTATCTTTAAGCAAAGTACAAATAAGTGAAAAGCTAAG
GAATATTTAAAAAGAAGAAAAAGCAGAGATTCCACACAACGTCAGAAAAAACCAGAGTTT
GGTTTCCCTGGACAATGTCTTTTTCTTTTAGTCTACTTTATTAGATGCCATTTTCATTGA
GTAAACAAACATTATTGTGAGATTATTAGAAAGAGACACTGTATAAAGGATAAAACAAAA
AGGCATACAAAGACAAGAACACTCATGAGCTTTTCCCTGCAGCCAGAGCTTCTTTTTTCA
TCACAGCTTTGACATAGATTTCATAGCAACATCCTAAGACAATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTC
Primers used for PCR MJ28 - 8474, show primer details
   
Sequence Name 25-K029911-022-373-H03C-8409
GenBank Accession LN497312 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K029911-022-373-H03C-8409
TCCATAATTCATATTACCAAGAAACAACAGAGAAGAACGTTGTTAGTTTGATTTGACATA
ATAAGCTCAGCTCTACATGAAACCTATGAGATTCAATCTACAGCTTTCTTAATTGCAAGA
AGCAAGGTGAAAATATCTAAGTGTGCCTAGCCTAGTCAATCTCTTACTCAGATTCATTAT
GATTCCTGAACCATACCAGATTATGTTTCTAGACCACTAACAAAACAAGAATCTGATTAG
CTTCGACCAAAAGTTATGAACTAAAGTGTTTTTACCTGCAACAACAACAACAGTGAGCAC
ATCACCAATCCTCTTCAGTTTGTGCACACTTTTCTTATGAGACTTGAATCTCTTCAAAGC
AGAATCTTCAGCCAAGAGCTACACAGGATTTCAATTTAAGCAAACACAAACTCATCAAGA
GCTTTAAGAAAATGCCATATAACAACAGCA
   
Sequence Name 25-K029911-022-373-H03C-MJ28
GenBank Accession LN497313 [GenBank]
Sequencing primer gene specific primer, MJ28: 5'-GTTGTTGATGCTTATGCTGTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K029911-022-373-H03C-MJ28
TGCTAAATTGAAATCCTGTGTAGCTCTTGGCTGAAGATTCTGCTTTGAAGAGATTCAAGT
CTCATAAGAAAAGTGTGCACAAACTGAAGAGGATTGGTGATGTGCTCACTGTTGTTGTTG
TTGCAGGTAAAAACACTTTAGTTCATAACTTTTGGTCGAAGCTAATCAGATTCTTGTTTT
GTTAGTGGTCTAGAAACATAATCTGGTATGGTTCAGGAATCATAATGAATCTGAGTAAGA
GATTGACTAGGCTAGGCACACTTAGATATTTTCACCTTGCTTCTTGCAATTAAGAAAGCT
GTAGATTGAATCTCATAGGTTTCATGTAGAGCTGAGCTTATTATGTCAAATCAAACTAAC
AACGTTCTTCTCTGTTGTTCTTGGTAATATGAATTATGGATTCACGACATACTCGAAATC
TAAATTGTGACTTATCTCGTGTCTTAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGT
ACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAA
ATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11