Sequences from confirmation amplicon


Confirmation sequences for line 374G03 and hit At2g21130 are shown.
Line ID 374G03
Locus At2g21130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9055636
Primers used for PCR JK30 - 8474, show primer details
   
Sequence Name 17-K024845-022-374-G03C-8409
GenBank Accession LN493699 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K024845-022-374-G03C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATTGACTATAGTTTAAATCACAATC
AGCGATCACAACAGGCTTCGTCGGCTTTCCAGAAGAAGATCCGATCTTCTCAATAGCCTT
AACAACGTCCAAGCCTTCAACCACCTGACCAAACACCACATGCTTACCATCAAGCCAATC
AGTCTTCACCGTACAGATGAAAAACTGAGATCCATTAGTGTTAGCACCAGCGTTAGCCAT
CGACAAAATCCCAGGTCCTGTGTGCTTCCTCTCGAAATTCTCATCCTCAAATTTGGCTCC
GTAGATCGATTCGCCACCAGTTCCGTTACCTTTGGTGAAATCTCCTCCTTGGCACATGAA
ATTTGGGATCACGCGGTGAAACGAAGATCCTTTGAAGTGAAGTGGCTTTCCGCTACGTCC
GACGCCTTTCTCTCCGGTGCATAGAGCTCTGAAATTCTCCGCCGTCTTTGGTGTCTTGTC
GGTGTAAAGCTCCATCACGATCTTCCCCGCTGGTGCACCGCCGATGGTCATGTCG
   
Sequence Name 18-K024845-022-374-G03C-JK30
GenBank Accession LN494147 [GenBank]
Sequencing primer gene specific primer, JK30: 5'-CCTAAAGTTTTCTTCGACATGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K024845-022-374-G03C-JK30
GCGGTGCACCAGCGGGGAAGATCGTGATGGAGCTTTACACCGACAAGACACCAAAGACGG
CGGAGAATTTCAGAGCTCTATGCACCGGAGAGAAAGGCGTCGGACGTAGCGGAAAGCCAC
TTCACTTCAAAGGATCTTCGTTTCACCGCGTGATCCCAAATTTCATGTGCCAAGGAGGAG
ATTTCACCAAAGGTAACGGAACTGGTGGCGAATCGATCTACGGAGCCAAATTTGAGGATG
AGAATTTCGAGAGGAAGCACACAGGACCTGGGATTTTGTCGATGGCTAACGCTGGTGCTA
ACACTAATGGATCTCAGTTTTTCATCTGTACGGTGAAGACTGATTGGCTTGATGGTAAGC
ATGTGGTGTTTGGTCAGGTGGTTGAAGGCTTGGACGTTGTTAAGGCTATTGAGAAGATCG
GATCTTCTTCTGGAAAGCCGACGAAGCCTGTTGTGATCGCTGATTGTGATTTAAACTATA
GTCAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATG

Last Updated on 06.12.2018 16:11