Sequences from confirmation amplicon


Confirmation sequences for line 376G12 and hit At1g04610 are shown.
Line ID 376G12
Locus At1g04610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1280845
Primers used for PCR ZZ65 - 8474, show primer details
   
Sequence Name 41-K021853-022-376-G12C-8409
GenBank Accession LN503923 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K021853-022-376-G12C-8409
TGAGCCATTTACATTGAATATATCCTGACATCATTAAATGTCTAAAATTTAAGCTAAACC
TTGAGAAGAAACAATGAATGAAAGATGTTTTATTTATTACCTTAAGCCATGAAGGGACGT
TGCTTCTGTATCCAGTAGCTAGAATAACGGAATCAATCTCAAGAACACGGCCATCTATTA
GCTCAACTTTGCCTTTGCCGAACTTTATGATTCCGGGGACGATTTTGATCTTTCCCGATC
TGATTTTGGGTAACGCTCCGATGTCAAGAACCGGAGTTTTGCCTTCCTTGTTCTTTAGCT
CTAACGGTCCAATTTTCGGCCTTTTTAGACCGTATTTATCGGTATTCCCCAAGATAATCC
TCGCCAGAAAGAGTATAGTCTTGTCCGCGAGCCAAACGGGCATATACTTCATCATCGTAA
CTCCTAATTCAAACGTTGATTTCCCAAAAATCTCTCTTGGTAAAACATGAACCTGATAAA
ATCAAGAAAGGAAAAAAAAACACAGACTATTAGATACTGCTCTGTTTTAGAAAACAGAGT
AACAGAAACAGAGGATGCTCTGTTTT
   
Sequence Name 42-K021853-022-376-G12C-ZZ65
GenBank Accession LN504312 [GenBank]
Sequencing primer gene specific primer, ZZ65: 5'-TTATAGCCCCTAAAACAGAGCATC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K021853-022-376-G12C-ZZ65
ACAGAGCAGTATCTAATAGTCTGTGTTTTTTTTTCCTTTCTTGATTTTATCAGGTTCATG
TTTTACCAAGAGAGATTTTTGGGAAATCAACGTTTGAATTAGGAGTTACGATGATGAAGT
ATATGCCCGTTTGGCTCGCGGACAAGACTATACTCTTTCTGGCGAGGATTATCTTGGGGA
ATACCGATAAATACGGTCTAAAAAGGCCGAAAATTGGACCGTTAGAGCTAAAGAACAAGG
AAGGCAAAACTCCGGTTCTTGACATCGGAGCGTTACCCAAAATCAGATCGGGAAAGATCA
AAATCGTCCCCGGAATCATAAAGTTCGGCAAAGGCAAAGTTGAGCTAATAGATGGCCGTG
TTCTTGAGATTGATTCCGTTATTCTAGCTACTGGATACAGAAGCAACGTCCCTTCATGGC
TTAAGGTAATAAATAAAACATCTTTCATTCATTGTTTCTTCTCAAGGTTTAGCTTAAATT
TTAGACATTTAATGATGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACT

Last Updated on 06.12.2018 16:11