Sequences from confirmation amplicon


Confirmation sequences for line 377B04 and hit At1g15690 are shown.
Line ID 377B04
Locus At1g15690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5402459
Primers used for PCR JZ53 - 8474, show primer details
   
Sequence Name 47-K038035-022-377-B04C-8409
GenBank Accession LN506130 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K038035-022-377-B04C-8409
CCATAAGCAGCAGAAAGTAACGGCTAAAACAAGTCTCGACCGAATACTTNGGTGGATTTC
TAAATTCTAAACAGAACAAAAAGAAAACAGAAAGACAGAGAGTGAGAGAAAGAAACATCA
TTTGGTTGGTTCTTTAAATGAGCCTCATCAACAAAAAAGGTTTTTTAACCACCTACGGTA
AACGAAAATAAGAATGGTAAAAAAGAAAGTTTAGAATCGATCGCCATCATCATCTTCTTC
TTCTTCATCATCGTCATCTTCTTCCCTCGGATTGAGTTTAGAAGTACTTGAAAAGGATAC
CACCGTGAGTGGCGAAGAAGGGAGCAAAGACAAGAGACTCAACAGCCATGAGCTTGATGA
GGATGTTCAATGAAGGTCCTGAAGTATCCTTCAATGGGTCTCCAATTGTGTCTCCAATCA
CAGCTGCCTTGTGTGGCTCTGAACCCTTTGGTCCAAGGCTCTTTGCGTGCTCTGATACAC
CAGCCTGCGAATATAT
   
Sequence Name 07-K038035-022-377-B04C-JZ53
GenBank Accession LN489186 [GenBank]
Sequencing primer gene specific primer, JZ53: 5'-AAGCATTGTTATATATTCGCAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K038035-022-377-B04C-JZ53
CTTGGACAAGGGTTCAGAGCCACACAAGGCAGCTGTGATTGGAGACACAATTGGAGACCC
ATTGAAGGATACTTCAGGACCTTCATTGAACATCCTCATCAAGCTCATGGCTGTTGAGTC
TCTTGTCTTTGCTCCCTTCTTCGCCACTCACGGTGGTATCCTTTTCAAGTACTTCTAAAC
TCAATCCGAGGGAAGAAGATGACGATGATGAAGAAGAAGAAGATGATGATGGCGATCGAT
TCTAAACTTTCTTTTTTACCATTCTTATTTTCGTTTACCGTAGGTGGTTAAAAAACCTTT
TTTGTTGATGAGGCTCATTTAAAGAACCAACCAAATGATGTTTCTTTCTCTCACTCTCTG
TCTTTCTGTTTTCTTTTTGTTCTGTTTAGAATTTAGAAATCCACCAAGTATTCGGTCGAG
ACTTGTTTTAGCCGTTACTTTCTGCTGCTTATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTA

Last Updated on 06.12.2018 16:11