Sequences from confirmation amplicon


Confirmation sequences for line 379C07 and hit At5g04180 are shown.
Line ID 379C07
Locus At5g04180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1148485
Primers used for PCR 51ra - 8474, show primer details
   
Sequence Name 23-K115806-0022-379-C07C-51ra
GenBank Accession LN496693 [GenBank]
Sequencing primer gene specific primer, 51ra: 5'-TGTTCTCCTGATATTTTGCAGATG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K115806-0022-379-C07C-51ra
CAACCCGGTGAGATGCCGATCCCTCGAAATGGAGCAGTATCAAGGCTGAATGGAAAATTT
GCGGGACAGGGAAGAGGCAATCGCCAATCAATCTTACTCCAAAAATAGCTCGCATTGTTC
ACAATTCTACAGAGATTCTTCAGACATATTACAAACCTGTAGAGGCTATTCTTAAGAACC
GTGGATTCGACATGAAGGTTAAGTGGGAAGACGATGCAGGGAAGATCGTGATCAATGATA
CCGACTATAAATTGGTTCAAAGCCACTGGCACGCACCTTCAGAGCATTTTCTCGATGGAC
AGAGGTAAAGGGAAAACAAACCCAAAGATTGACATCATTTCTGATATATATATATAGTAG
CTACGAGCTAATAAATATCAAACTTGGATTTGGTTTTAGGTTGGCAATGGAACTTCACAT
GGTACACAAAAGTGTAGAAGGGCACTTGGCAGTGATTGGAGTTCTCTTCAGAGAAGGAGA
ACCAAATGCTTTCATTTCGCGGGTAATGTTAACCGCGATTGCATTTACCATACCGTTACA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 02-K115805-0022-379-C07C-8409
GenBank Accession LN487174 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115805-0022-379-C07C-8409
TACGGTATGGTAATGCAATCNGCGGTTAACATTACCCGCGAAATGAAAGCATTTGGTTCT
CCTTCTCTGAAGAGAACTCCAATCACTGCCAAGTGCCCTTCTACACTTTTGTGTACCATG
TGAAGTTCCATTGCCAACCTAAAACCAAATCCAAGTTTGATATTTATTAGCTCGTAGCTA
CTATATATATATATCAGAAATGATGTCAATCTTTGGGTTTGTTTTCCCTTTACCTCTGTC
CATCGAGAAAATGCTCTGAAGGTGCGTGCCAGTGGCTTTGAACCAATTTATAGTCGGTAT
CATTGATCACGATCTTCCCTGCATCGTCTTCCCACTTAACCTTCATGTCGAATCCACGGT
TCTTAAGAATAGCCTCTACAGGTTTGTAATATGTCTGAAGAATCTCTGTAGAATTGTGAA
CAATGCGAGCTATTTTTGGAGTAAGATTGATTGGCGATTGCCTCTTCCCTGTCCCGCAAA
TTTTCCATTCAGCCTTGATACTGCTCCATTTCGAGGGATCGGCTATCTCACCGGGTTTGT
AATGAAATTCAGTCTCTGTCTCATCTGCAAAAT

Last Updated on 06.12.2018 16:11