Sequences from confirmation amplicon


Confirmation sequences for line 379F02 and hit At5g05410 are shown.
Line ID 379F02
Locus At5g05410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1602671
Primers used for PCR RD04 - 8474, show primer details
   
Sequence Name 03-K027788-022-379-F02C-8409
GenBank Accession LN487342 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K027788-022-379-F02C-8409
AGCCATTTACATTGAATAATTCACTCTGATCATAAACTGCCATCTCCTTCCCAGAAACAA
CACAATCTACAATACAACAACACTTCAATATCAGAACACAAGCTGAAACATAAGGCAAGA
GAACTTAAACTTGGTTCAAAATCAGATCGATTTACTTATTTGTTATCAAATTAGGAACAC
CTAAAATTTCCCAATTTCACAGCTATACCTAAAAGGAAATAATCGAAAACCTAAAGACGA
AGATAAACCCTAGAATCACCAATTGACGAGACCTAATCAGAGAAATTAATTGAAAATTTG
TAAGAAATCAGAAATCAACGTACCTATAGGGGACGAAATTTGAAAATCAAAGTCTCTTCC
TTCGAGTTGATAAATCGCTTTCGTGTTTAGTCTTCTTGCTCGTATCTNTTCTTCTTCTTC
TT
   
Sequence Name 04-K027788-022-379-F02C-RD04
GenBank Accession LN487780 [GenBank]
Sequencing primer gene specific primer, RD04: 5'-CGAAAAAGCTACACACAAGAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K027788-022-379-F02C-RD04
ACACGAAGCGATTTATCAACTCGAAGGAAGAGACTTTGATTTTCAATTTCGTCCCCTATA
GGTACGTTGATTTCTGATTTCTTACAAATTTTCAATTAATTTCTCTGATTAGGTCTCGTC
AATTGGTGATTCTAGGGTTTATCTTCGTCTTTAGGTTTTCGATTATTTCCTTTTAGGTAT
AGCTGTGAAATTGGGAAATTTTAGGTGTTCCTAATTTGATAACAAATAAGTAAATCGATC
TGATTTTGAACCAAGTTTAAGTTCTCTTGCCTTATGTTTCAGCTTGTGTTCTGATATTGA
AGTGTTGTTGTATTGTAGATTGTGTTGTTTCTGGGAAGGAGATGGCAGTTTATGATCAGA
GTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACTTC
Primers used for PCR FN43 - 8474, show primer details
   
Sequence Name 16-K035333-022-379-F02C-8409
GenBank Accession LN493344 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K035333-022-379-F02C-8409
TGAGCCATTTACAATTGCATGTAGAACACAAATTGATACATCGAGGAAAAGGAAATCTAG
AAGTAGAGGTGACGGTACTACTGTGGCTGAGAGATTAAAGAGATGGAAAGAGTATAACGA
GACCGTAGAAGAAGTTTCTACCAAGAAGAGGAAAGTACCTGCGAAAGGGTCGAAGAAGGG
TTGTATGAAAGGTAAAGGAGGACCAGAGAATAGCCGATGTAGTTTCAGAGGAGTTAGGCA
AAGGATTTGGGGTAAATGGGTTGCTGAGATCAGAGAGCCTAATCGAGGTAGCAGGCTTTG
GCTTGGTACTTTCCCTACTGCTCAAGAA
   
Sequence Name 16-K035333-022-379-F02C-FN43
GenBank Accession LN493345 [GenBank]
Sequencing primer gene specific primer, FN43: 5'-GCTTCTTGAGCAGTAGGGAAAGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K035333-022-379-F02C-FN43
TCGATAGGCTCTCTGATCTCGCACCCATTTACCCCAAATCCTTTGCCTAACTCCTCTGAA
ACTACATCGGCTATTCTCTGGTCCTCCTTTACCTTTCATACAACCCTTCTTCGACCCTTT
CGCAGGTACTTTCCTCTTCTTGGTAGAAACTTCTTCTACGGTCTCGTTATACTCTTTCCA
TCTCTTTAATCTCTCAGCCACAGTAGTACCGTCACCTCTACTTCTAGATTTCCTTTTCCT
CGATGTATCAATTTGTGTTCTACATGCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGGA

Last Updated on 06.12.2018 16:11