Sequences from confirmation amplicon


Confirmation sequences for line 381F11 and hit At3g15950 are shown.
Line ID 381F11
Locus At3g15950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5400011
Primers used for PCR JE25 - 8474, show primer details
   
Sequence Name 34-K101564-0022-381-F11C-8409
GenBank Accession LN501356 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K101564-0022-381-F11C-8409
CATTTACATTGAAGCCATGTAGATTTAAAAATCTTGAATTATTTCCTCTGTTTATCAAAC
TACTTTGCAGCTGCTACAAATAGTCTTAAGCAACTAAACCTTGACGATTTCAGTGAAGGA
GATGACAGTGCAGAATGTAATAACTTGTCCTCTCTACTTTCTCTGATCATATTTGCATCT
TCTATAGTATTTTGAAGATTTTTTTGTATGTTTTGATACAGCTGCAAGGAGAAATAGTAT
GCTTGAAGCTATCGAACGCGAGTTTGAAGGTCATTTTCACATAACATTGAACTTCCTAGC
TGAAATGTAAATCCATTCTAAAAGGTCAAGTACTTAAGTTTCTTCTCTTTTATTTTCTTA
CTCGCAGCTGCTACAAAAGGTCTTGAAGAGCTAAAGGCTAATGATTCAACCGGCGACAAG
GATGATGATGAACACGGTAACTATTCTTCCCCATATTCCTCTATTTTGTATATATTGTTC
TGTTGTATCCTGAGCCAGCTCTGTTTCCATGTTTTGTTACAGTTGCAAGGAGAAAAATTA
TGCTTGAAGCTATTGAACGCGAGTTTGAAGGTACTTTAACATATATTCTTGAGCCATTGT
TGGTGACCAAGCCTTGATAACTGANTAAATCGG
   
Sequence Name 34-K101577-0022-381-F11C-JE25
GenBank Accession LN501357 [GenBank]
Sequencing primer gene specific primer, JE25: 5'-CCGATTCATCTAGTTATCAAGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K101577-0022-381-F11C-JE25
GATATTGTTAAGTCCTTCAAACTCGCGTTCATAGCTTCAAGCATAATTTTTCTCCTTGCA
ACTGTAACAAAACATGGAAACAGAGCTGGCTCAGGATACAACAGAACAATATATACAAAA
TAGAGGAATATGGGGAAGAATAGTTACCGTGTTCATCATCATCCTTGTCGCCGGTTGAAT
CATTAGCCTTTAGCTCTTCAAGACCTTTTGTAGCAGCTGCGAGTAAGAAAATAAAAGAGA
AGAAACTTAAGTACTTGACCTTTTAGAATGGATTTACATTTCAGCTAGGAAGTTCAATGT
TATGTGAAAATGACCTTCAAACTCGCGTTCGATAGCTTCAAGCATACTATTTCTCCTTGC
AGCTGTATCAAAACATACAAAAAAATCTTCAAAATACTATAGAAGATGCAAATATGATCA
GAGAAAGTAGAGAGGACAAGTTATTACATTCTGCACTGTCATCTCCTTCACTGAAATCGT
CAAGGTTTAGTTGCTTAAGACTATTTGTAGCAGCTGCAAAGTAGTTTGATAAACAGAGGA
AATAATTCAAGATTTTTAAATCTACATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATATCCG
Primers used for PCR JL28 - 8474, show primer details
   
Sequence Name 66-K101717-0022-381-F11C-8409
GenBank Accession LN511566 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K101717-0022-381-F11C-8409
CGGACTGAGCCATTTACAATTGAAGCCTGTAGATTTAAAAATCTTGAATTATTTCCTCTG
TTTATCAAACTACTTTGCAGCTGCTACAAATAGTCTTAAGCAACTAAACCTTGACGATTT
CAGTGAAGGAGATGACAGTGCAGAATGTAATAACTTGTCCTCTCTACTTTCTCTGATCAT
ATTTGCATCTTCTATAGTATTTTGAAGATTTTTTTGTATGTTTTGATACAGCTGCAAGGA
GAAATAGTATGCTTGAAGCTATCGAACGCGAGTTTGAAGGTCATTTTCACATAACATTGA
ACTTCCTAGCTGAAATGTAAATCCATTCTAAAAGGTCAAGTACTTAAGTTTCTTCTCTTT
TATTTTCTTACTCGCAGCTGCTACAAAAGGTCTTGAAGAGCTAAAGGCTAATGATTCAAC
CGGCGACAAGGATGATGATGAACACGGTAACTATTCTTCCCCATATTCCTCTATNTTGTA
TATNTTGTTCTGTTGTATCCNGAGCCAGCTCTGTTTCCATGTTTTGTTAC
   
Sequence Name 66-K101724-0022-381-F11C-JL28
GenBank Accession LN511567 [GenBank]
Sequencing primer gene specific primer, JL28: 5'-TGTAACAAAACATGGAAACAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K101724-0022-381-F11C-JL28
ACATATTACAAATAGAGGAATATGGGGAAGATAGTTACCGTGTTCATCATCATCCTTGTC
GCCGGTTGAATCATTAGCCTTTAGCTCTTCAAGACCTTTTGTAGCAGCTGCGAGTAAGAA
AATAAAAGAGAAGAAACTTAAGTACTTGACCTTTTAGAATGGATTTACATTTCAGCTAGG
AAGTTCAATGTTATGTGAAAATGACCTTCAAACTCGCGTTCGATAGCTTCAAGCATACTA
TTTCTCCTTGCAGCTGTATCAAAACATACAAAAAAATCTTCAAAATACTATAGAAGATGC
AAATATGATCAGAGAAAGTAGAGAGGACAAGTTATTACATTCTGCACTGTCATCTCCTTC
ACTGAAATCGTCAAGGTTTAGTTGCTTAAGACTATTTGTAGCAGCTGCAAAGTAGTTTGA
TAAACAGAGGAAATAATTCAAGATTTTTAAATCTACATGGCTTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGC

Last Updated on 06.12.2018 16:11