Sequences from confirmation amplicon


Confirmation sequences for line 382E01 and hit At1g48000 are shown.
Line ID 382E01
Locus At1g48000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17705840
Primers used for PCR RS11 - 8474, show primer details
   
Sequence Name 12-K018976-022-382-E01C-RS11
GenBank Accession LN491368 [GenBank]
Sequencing primer gene specific primer, RS11: 5'-CTTCAACGATATGTGTCCCTTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K018976-022-382-E01C-RS11
GTAATGTATCGACAATATGACTATGTCTATGTCGATATAAATAAAGGTTTCATACCGATT
TCCCCAACGAGAATGGAGTTCAAGGATGATAAATTGTTCTTGAAGGGATATGTCTCCACG
GCGGATATCCGGCCGGAGATAATTTACCCACCGCAATCTGCAACTTTTCCCCGTTTCTAT
TCAGCCCTGTCTCAATAGTAAATGATTTCAATATAAATACAATTAGTATAAAAACATGAA
TTAACTGGAATGTACACTAGTCAATAGTATATGGACACTAGTACAATTTCCCAAATCAAT
AAAGATAACAATTCCATGAGGCTGTATGGAATTGGATGGTTGGGAAAGGTGTTTGAGTTC
CACACTCAAAAGGCTAATATGAAAAAAACTCCAAAGGAAAAAATAAATAAATTTGGACCA
ACCAAACTGTTTCTATACAAAAGTACACTTTTAATGATAATAATGAACTATGAATATGTC
AACTTGTTCTTTCTTACTTTACCAATTCTACAGAGGAATTTAAATTATGCCGACATGGAG
AAGTCACAGCCATAAGAAAAGTATTCTCATAGTTACGAAAAATCACATTCATATATGTAT
ATCACTCACATATGATGTGATAGAAATTGAATAACATATTGATAAGATGTGGACGAGTTT
TTATATCTACTCCATTGAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAA
   
Sequence Name 16-K018976-022-382-E01C-8409
GenBank Accession LN493234 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K018976-022-382-E01C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTCAATGGAGTAGATATAAAAACTC
GTCCACATCTTATCAATATGTTATTCAATTTCTATCACATCATATGTGAGTGATATACAT
ATATGAATGTGATTTTTCGTAACTATGAAAATACTTTTCTTA

Last Updated on 06.12.2018 16:11