Sequences from confirmation amplicon


Confirmation sequences for line 382H01 and hit At3g54250 are shown.
Line ID 382H01
Locus At3g54250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20084590
Primers used for PCR HV14 - 8474, show primer details
   
Sequence Name 15-K031867-022-382-H01C-8409
GenBank Accession LN492857 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K031867-022-382-H01C-8409
CATTAGCGTAACTCTTGATCCTGATCATCTCTGCACAGTCACCACTGTTGCCGTTAGTCC
TGCTTTTGATCGGGATCGAATGTGGCTCAATGGCAAGGTACACTCGCTTCATTTCCCATC
ACGATCTCTCTCCTTTTGCTGAGATTCCAATTATCGAATTACAAAGATTTTTGTTGTTGT
TGTTGTTTCATGTTAGTGTAGGAAATATCTCTTTCTGGAAGTAGGTACCAAAATTGTTTG
AGGGAAATACGAGGTCGTGCTGGTGATGTTGAAGACATGGAAAAGGGGATCAAGATAAGG
AAGAAAGATTGGGAGAAATTGAATCTACATATTGCTTCTCATAATAACTTCCCTACTGCT
GCTGGCTTAGCATCTTCAGCTGCTGGATTTGCTTGTCTAGGTATTCTGCTTTTTCTCTCG
NTTGATG
   
Sequence Name 16-K031867-022-382-H01C-HV14
GenBank Accession LN493324 [GenBank]
Sequencing primer gene specific primer, HV14: 5'-TCATCAACAGAGAGAAAAAGCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K031867-022-382-H01C-HV14
CAGCTGAGATGCTAAGCCAGCAGCAGTAGGGAAGTTATTATGAGAAGCAATATGTAGATT
CAATTTCTCCCAATCTTTCTTCCTTATCTTGATCCCCTTTTCCATGTCTTCAACATCACC
AGCACGACCTCGTATTTCCCTCAAACAATTTTGGTACCTACTTCCAGAAAGAGATATTTC
CTACACTAACATGAAACAACAACAACAACAAAAATCTTTGTAATTCGATAATTGGAATCT
CAGCAAAAGGAGAGAGATCGTGATGGGAAATGAAGCGAGTGTACCTTGCCATTGAGCCAC
ATTCGATCCCGATCAAAAGCAGGACTAACGGCAACAGTGGTGACTGTGCAGAGATGATCA
GGATCAAGAGTTACGCTAATGCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGA
TCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTT
CGACGTGTCTACATTCA
Primers used for PCR FS09 - 3144, show primer details
   
Sequence Name 53-K033041-022-382-H01C-3144
GenBank Accession LN508060 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K033041-022-382-H01C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAGAATCCGAA
CCTCATCTCTCTTTCCCCAATACTTAATCACCGCAATGTTCGTCGGAGTTTGCGCCGTCA
CCATGAACACCCATTTCTCCGTCGCCATGTGAATCTCCAAAAGAGATCTGCAGCTTCAAA
GTAGTTGAAAATGAAAGCGACGGAGAATACGACAGAATAATGATGACTCATTGCCTTTAC
AATTTTAAAAATATTATTTTATTCAAAAATTGAGAACTTCACTAACTGTAAAACTCTAAT
CAAATGGTTATTGCCATTTTGATGAAAACACCAAAACTTAACCTAAAACGTTTTAGGAAA
AAAATTGAGTTAGGTAAGAAATAACCAAAACACGAAAATCATTGTGAGCAAATGAAC
   
Sequence Name 21-K033041-022-382-H01C-FS09
GenBank Accession LN495566 [GenBank]
Sequencing primer gene specific primer, FS09: 5'-AGTTCATGCATCACAATGATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K033041-022-382-H01C-FS09
TACTCATTTTTTTCCTAAACGTTTTAGGTAAGTTTTGGTGTTTTCATCAAAATGGCAATA
ACCATTTGATTAGAGTTTTACAGTTAGTGAAGTTCTCAATTTTTGAATAAAATAATATTT
TTAAAATTGTAAAGGCAATGAGTCATCATTATTCTGTCGTATTCTCCGTCGCTTTCATTT
TCAACTACTTTGAAGCTGCAGATCTCTTTTGGAGATTCACATGGCGACGGAGAAATGGGT
GTTCATGGTGACGGCGCAAACTCCGACGAACATTGCGGTGATTAAGTATTGGGGAAAGAG
AGATGAGGTTCGGATTCTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTC
TTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATC
CACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGT

Last Updated on 06.12.2018 16:11