Sequences from confirmation amplicon


Confirmation sequences for line 383B01 and hit At2g36970 are shown.
Line ID 383B01
Locus At2g36970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15530635
Primers used for PCR JA24 - 8474, show primer details
   
Sequence Name 21-K100938-0022-383-B01C-8409
GenBank Accession LN495585 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K100938-0022-383-B01C-8409
GCCATTTACAAGTTAAAATTCGTCTCCGAAGATCAACGGTTGTAACCGCATCTTTGAGAT
GACGTTTAACCTTTTCAACATTGTTTCTTAGCTCACTTGAAGTTTCTCCATTCATCAATC
TTTTAACATTCGCTGAGACTTGGTCCCTTGTGATTGTCTTCTTCTCACAAAGATTAATCC
CAATGCACCAATCATCGACCACAAGCTTCCTATTCGTGAACTGATCTGTCAAAAGTGGAT
AACACAACAAAGGCAAACCACACCAAACGCTCTCTAGAATTGAATTCCATCCACAATGTG
TGAAAAACCCTCCCACGGCCGGATTTGAAATAACTTCCATCTGGCAGCACCATTGGACCA
CAAGACCTCGATCTTGGGCTTGGTCCACAAACCCGGCTGGAAGAAAATCTGGTACGTTGG
ATCCAACTATATCCGGACGTAAAACCCAATTGAAACT
   
Sequence Name 21-K100944-0022-383-B01C-JA24
GenBank Accession LN495586 [GenBank]
Sequencing primer gene specific primer, JA24: 5'-TAGTTTCATTTGGGTTTTACGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K100944-0022-383-B01C-JA24
ATTTTCTTCCGCCGGGTTTGTGGACCAGCCCAAGATCGAGGTCTTGTGGTCCAATGGTGC
TGCCAGATGGAAGTTATTTCAAATCCGGCCGTGGGAGGGTTTTTCACACATTGTGGATGG
AATTCAATTCTAGAGAGCGTTTGGTGTGGTTTGCCTTTGTTGTGTTATCCACTTTTGACA
GATCAGTTCACGAATAGGAAGCTTGTGGTCGATGATTGGTGCATTGGGATTAATCTTTGT
GAGAAGAAGACAATCACAAGGGACCAAGTCTCAGCGAATGTTAAAAGATTGATGAATGGA
GAAACTTCAAGTGAGCTAAGAAACAATGTTGAAAAGGTTAAACGTCATCTCAAAGATGCG
GTTACAACCGTTGGATCTTCGGAGACGAATTTTAACTTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCC

Last Updated on 06.12.2018 16:11