Sequences from confirmation amplicon


Confirmation sequences for line 385F02 and hit At4g23160 are shown.
Line ID 385F02
Locus At4g23160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12131797
Primers used for PCR AL01 - 8474, show primer details
   
Sequence Name 19-K022407-022-385-F02C-8409
GenBank Accession LN494578 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K022407-022-385-F02C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAAAATGTATTCTCTCTGATTTGGACACC
GAGTTAACGATTCGTTAACGGAGAAGGCAACGCAGTTACTGCAAACTTCCGGGGATAGGT
CTCCGCGACAAAGGAAAAGTCCGGTGACCCTGTCGGGGGTTTGTCCCGCCCTGATGTTTT
GGAATCCGGTGGAGTAAGAGGCGTTGCGGGAAGCAAAAGAGGACAAAAGGGTTTTGAGAT
TGGTGGAGTAAGTGCTGTTACTTGAGTAAGTTGTCCTATTTGGACAATCATGATTTAGGT
AAAAAGGATTTTGAGCAGAAGCTTTGAAGCTAGTGAGGAATGAGAAAAGGAAAAGGAAAA
T
   
Sequence Name 20-K022407-022-385-F02C-AL01
GenBank Accession LN495034 [GenBank]
Sequencing primer gene specific primer, AL01: 5'-TTCATTTTCCTTTTCCTTTTCTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K022407-022-385-F02C-AL01
AGATTTCCCGTTCCTCACTAGCTTCAAAGCTTCTGCTCAAAATCCTTTTTACCTAAATCA
TGATTGTCCAAATAGGACAACTTACTCAAGTAACAGCACTTACTCCACCAATCTCAAAAC
CCTTTTGTCCTCTTTTGCTTCCCGCAACGCCTCTTACTCCACCGGATTCCAAAACATCAG
GGCGGGACAAACCCCCGACAGGGTCACCGGACTTTTCCTTTGTCGCGGAGACCTATCCCC
GGAAGTTTGCAGTAACTGCGTTGCCTTCTCCGTTAACGAATCGTTAACTCGGTGTCCAAA
TCAGAGAGAATACATTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATTCACG

Last Updated on 06.12.2018 16:11