Sequences from confirmation amplicon


Confirmation sequences for line 386A08 and hit At2g34470 are shown.
Line ID 386A08
Locus At2g34470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14531797
Primers used for PCR HM04 - 8474, show primer details
   
Sequence Name 13-K028846-022-386-A08C-8409
GenBank Accession LN491900 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K028846-022-386-A08C-8409
ATGAAGCCATTTACAATTGAATATAGATTTACGGATTTGGGTTTCATTTACATGTAGATC
GTGAGTATTATTATCAGTGGCAAACACAAGATTGTTTTCTCGATCTCACTCATGTGACTA
AATAAGATTTTGATATTGTAAACGTTGTAAGTGACAAATGATAT
   
Sequence Name 14-K028846-022-386-A08C-HM04
GenBank Accession LN492370 [GenBank]
Sequencing primer gene specific primer, HM04: 5'-CTAGAAATGAAACCTCACCTCCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K028846-022-386-A08C-HM04
CAAAGAAGCAAATCAGCCTTGAATAAGTTAGATAACTCCTCAAGAGGACCCAAATTGATA
CTGATATCTTCACGGATAGCAGCGTGTGGACATCCACCTGTTTCAACAGCTCTAATCCTT
TCCTCAGGAAGAGCCCCATTCTTCACCAGAAACTCACCATCCTCTTTCGTGAATATATCA
TTTGTCACCTACAACGTTTACAATATCAAAATCTTATTTAGTCACATGAGTGAGATCGAG
AAAACAATCTTGTGTTTGCCACTGATAATAATACTCACGATCTACATGTAAATGAAACCC
AAATCCGTAAATCTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11