Sequences from confirmation amplicon


Confirmation sequences for line 387F09 and hit At3g55320 are shown.
Line ID 387F09
Locus At3g55320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20511364
Primers used for PCR KP68 - 8474, show primer details
   
Sequence Name 46-K034066-022-387-F09C-KP68
GenBank Accession LN505786 [GenBank]
Sequencing primer gene specific primer, KP68: 5'-CTTCCGACACTAGTTTATTGCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K034066-022-387-F09C-KP68
TTCTAATTCTTAGAAAGTTTATCTTAAATGTGCAGGCGATCTCTTACATTAGGACTTTAT
ATGCCTTTACAAATGAAACTCTAGCAAAATATTCATATGCAACCTCCCTTCAAGCAACCC
TGAGATACGGCATATTAATTAGTCTTGTGCAAGGCCTTGGACTTGGATTTACTTATGGGC
TTGCTATATGCTCGTGTGCGCTGCAACTTTGGATTGGACGATTCTTTGTTCACAATGGAA
GGGCAAATGGTGGAGAAATCAATTACCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAAATGG
   
Sequence Name 30-K034066-022-387-F09C-8409
GenBank Accession LN499623 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K034066-022-387-F09C-8409
AAGCCATTTACAATNTGGTAATTGATTTCTCCACCATTTGCCCTTCCATTGTGAACAAAG
AATCGTCCAATCCAAAGTTGCAGCGCACACGAGCATATAGCAAGCCCATAAGTAAATCCA
AGTCCAAGGCCTTGCACAAGACTAATTAATATGCCGTATCTCAGGGTTGCTTGAAGGGAG
GTTGCATATGAATATTTTGCTAGAGTTTCATTTGTAAAGGCATATAAAGTCCTAATGTAA
GAGATCGCCTGCACATTTAAGATAAATCTTTCTAAGAATTAGAATATTGACACCAGACTC
TAACTGAGGCAATAAACTAGTGGTCGGAAGACTAGAGA

Last Updated on 06.12.2018 16:11