Sequences from confirmation amplicon


Confirmation sequences for line 389A01 and hit F21B23 are shown.
Line ID 389A01
Locus F21B23 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:10372599
Primers used for PCR ZZ71 - 8474, show primer details
   
Sequence Name 43-K021853-022-389-A01C-8409
GenBank Accession LN504695 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K021853-022-389-A01C-8409
ATGAGCCATTGGTCATTGAAGCCATGTAGATAGAGAAAGGAAGAAAGGTGAGAAAGGGAG
ATTTAGAAAAAGGTAAACAAGAGAGAGAGAAAGGTGAGGAAGAGATATCAGAAAAGAGGT
AAACGAGAGAAAAAGAGAAAGGTAAGAGAGAGATAGAAGAAGAACATAAGCTTAGATATC
AACAATAACGAAAAACAATTAAGATGGACTCTTTTAAGCTCTGAATGACAATGTAAAAGC
ATCTTATGATCATTGAGTTAGGAAAAAAGAGTGTTATGTGAAACCTTAATTTGCATTCGA
CCACAATTTATTTAAAATACATAATACTTTTAAGTTCAAAACGATATTTGTACAAACATG
ATCACACACAAACGCATTCACCTCCCCATAACCCTTTTTCGTTGTCTCACTTGGGTGTGA
AAGCCTGTAAAAAAACACAAAAGCTAGTCATTTAAACGAGTGGATGTTATGAATATTTAA
AAAGATATGTTTTGATTCGGATCACACTTACAGCGAAGATGGTGGCGTGACCAGGATCAG
CAAGATGTGCAAAAAGGTTATC
   
Sequence Name 44-K021853-022-389-A01C-ZZ71
GenBank Accession LN505043 [GenBank]
Sequencing primer gene specific primer, ZZ71: 5'-GCACTGGTCCTATTGATAACCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K021853-022-389-A01C-ZZ71
CGCCCCATCTTCGCTGTAAGTGTGATCCGAATCAAAACATATCNTTTTTAAATATTCATA
ACATCCACTCGTTTAAATGACTAGCTTTTGTGTTTTTTTACAGTGCTTTCACACCCAAGT
GAGACAACGAAAAAGGGTTATGGGGAGGTGAATGCGTTTGTGTGTGATCATGTTTGTACA
AATATCGTTTTGAACTTAAAAGTATTATGTATTTTAAATAAATTGTGGTCGAATGCAAAT
TAAGGTTTCACATAACACTCTTTTTTCCTAACTCAATGATCATAAGATGCTTTTACATTG
TCATTCAGAGCTTAAAAGAGTCCATCTTAATTGTTTTTCGTTATTGTTGATATCTAAGCT
TATGTTCTTCTTCTATCTCTCTCTTACCTTTCTCTTTTTCTCTCGTTTACCTCTTTTCTG
ATATCTCTTCCTCACCTTTCTCTCTCTCTTGTTTACCTTTTTCTAAATCTCCCTTTCTCA
CCTTTCTTCCTTTCTCTATCTACATGGCTTCAATTGACAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11