Sequences from confirmation amplicon


Confirmation sequences for line 389C06 and hit At3g62190 are shown.
Line ID 389C06
Locus At3g62190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23021093
Primers used for PCR NK85 - 8474, show primer details
   
Sequence Name 14-K103453-0022-389-C06C-NK85
GenBank Accession LN492472 [GenBank]
Sequencing primer gene specific primer, NK85: 5'-CCCATACTTTCTTCCTGTAAGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K103453-0022-389-C06C-NK85
TCACACATGTATAAAATAAATCCACCAATCTCCCTCGCAATTCCCATACCTAACAAACAA
AACTTGTGGATATACCATCGATTCAACTAAAATTGACATCACCAAATTCGAGTCTATAAT
CGAACCATTTCAGCTCTCAAATTCAAAAGACAGATAGATTCAAAAACCCTAATTGATCGA
ATTCAGTGATCGAAAATCTCGCGGAGAAACTCACAAACGACCGTAATCGAAAAAGAAGAG
AAGAAGAAACCTGAGAAGGATCGGGACGAGAATTAGGAGGAAAGCCGAGCAAGATCTTGG
CTTCCTCCACCTCCATTTTCCAAAAAGCTTCCCGCGTCTACGGTTTCGCTTCAGAGAGTC
GGTCCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGGTTATT
   
Sequence Name 30-K103454-0022-389-C06C-8409
GenBank Accession LN499681 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K103454-0022-389-C06C-8409
GAGCCATTTACAATTGAAGGACCGACTCTCTGAAGCGAAACCGTAGACGCGGGAAGCTTT
TTGGAAAATGGAGGTGGAGGAAGCCAAGATCTTGCTCGGCTTTCCTCCTAATTCTCGTCC
CGATCCTTCTCAGGTTTCTTCTTCTCTTCTTTTTCGATTACGGTCGTTTGTGAGTTTCTC
CGCGAGATTTTCGATCACTGAATTCGATCAATTAGGGTTTTTGAATCTATCTGTCTTTTG
AATTTGAGAGCTGAAATGGTTCGATTATAGACTCGAATTTGGTGATGTCAATTTTAGTTG
AATCGATGGTATATCCACAAGTTTTGTTTGTTAGGTATGGGAATTGCGAGGGAGATTGGT
GGATTTATTTTAATACATGTGTTGATATGAATCGATGATAGGTTAAAGCTGCTTACAGGA
AGAAAGTATGGG

Last Updated on 06.12.2018 16:11