Sequences from confirmation amplicon


Confirmation sequences for line 390B09 and hit At1g76790 are shown.
Line ID 390B09
Locus At1g76790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28823788
Primers used for PCR CP43 - 8474, show primer details
   
Sequence Name 17-K031139-022-390-B09C-8409
GenBank Accession LN493735 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K031139-022-390-B09C-8409
ATTTACAATGGAACTCATTCTCGTATATAATATCCCTATCTCTTTTATTTTATTTTATTT
TAAATAGCAATAATTTCGTATGAAATATCTTTTACATCAAATATCTCTATAAGCCTCGTC
TTTAAAACCAGACACAGAAACTCCTCACTTTCGAAAAAAACAAGATCATTACACAAAAAT
GGGACACCTAATTCCTCAAACCGGAGATGAAGAGACCGAGCTTGGTTTAGCTGCGGTTAG
GCTAGCTAACTGTGCAGCCTTCCCAATGGTTTTCAAAGCTGCCATCGAGCTCGGTGTCAT
CGACACTCTCTACTTAGCTGCTCGTGATGACGTCACCGGATCCAGTTCCTTCCTCACACC
TTCTGAGATAGCTATTAGGCTCCCCACAAAACCTAGTAACCCTGAAGCACCAGCTCTGTT
GGATCGTATTCTTCGTTTACTTGCTAGCTACTCCATGGTCAAGTGCCAAATTACGAATGG
TAA
   
Sequence Name 52-K031139-022-390-B09C-CP43
GenBank Accession LN507708 [GenBank]
Sequencing primer gene specific primer, CP43: 5'-GTTACCATCGATAATTTGGCACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K031139-022-390-B09C-CP43
GTACGAGNAACGATCCAACAGAGCTGGTGCTTCAGGGTTACTAGGTTTTGTGGGGAGCCT
AATAGCTATCTCAGAAGGTGTGAGGAAGGAACTGGATCCGGTGACGTCATCACGAGCAGC
TAAGTAGAGAGTGTCGATGACACCGAGCTCGATGGCAGCTTTGAAAACCATTGGGAAGGC
TGCACAGTTAGCTAGCCTAACCGCAGCTAAACCAAGCTCGGTCTCTTCATCTCCGGTTTG
AGGAATTAGGTGTCCCATTTTTGTGTAATGATCTTGTTTTTTTCGAAAGTGAGGAGTTTC
TGTGTCTGGTTTTAAAGACGAGGCTTATAGAGATATTTGATGTAAAAGATATTTCATACG
AAATTATTGCTATTTAAAATAAAATAAAATAAAAGAGATAGGGATATTATATACGAGAAT
GAGTATCCATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTC

Last Updated on 06.12.2018 16:11