Sequences from confirmation amplicon


Confirmation sequences for line 392A09 and hit At3g20770 are shown.
Line ID 392A09
Locus At3g20770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7262723
Primers used for PCR NT43 - 8474, show primer details
   
Sequence Name 67-K036526-022-392-A09C-NT43
GenBank Accession LN511752 [GenBank]
Sequencing primer gene specific primer, NT43: 5'-AAATTATCAGAAGCACCAGTCACA-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K036526-022-392-A09C-NT43
TCCCATAAAAAAGCCTTGAGCTTTACAAACTTCCATCATCTTCAACATATACTTCAAGAT
CCCATCTTGAGCTCTAGACATTTTCTTCCTCCTAGCTTGCTCTTGAGACTGCCTCTGTTT
AGCAGCATCAACACCTTCTTTACCCTTATCCTGCTCCTTGAGACGTTTAAGCCGCATTTT
GTCTCTCCACATCCTCCTCTCCAATTCATCAACATCAATCTCATCATCAGTATAGTCATC
TTCAACAATGGAATCAGGCTCAGCTTGTGGAACAGGACAGAAATCAACTTCACCAAGTGA
TCCAGAAGAGAAGAAATCCATGTTTCCACACATTCCCATCTCATTAAACATCATTGTAAC
CTGTAACAAATCAAATACACAAACAAAACTAATCAGTACAATTATATTTCTTCTGAATCA
AACAACTTACTAAAAAAAATCATCAAACCCTAAATTATAAAAAAGGGGCTTTTCGAATTC
AATCAAAGTTTTAAGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
TTCACGCCCA
   
Sequence Name 43-K036526-022-392-A09C-8409
GenBank Accession LN504758 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K036526-022-392-A09C-8409
ACTTTGATTGATTCGAAAGCCCCTTTTTTATAATTTAGGGTTTGATGATTTTTTTTAGTA
AGTTGTTTGATTCAGAAGAAATATAATTGTACTGATTAGTTTTGTTTGTGTATTTGATTT
GTTACAGGTTACAATGATGTTTAATGAGATGGGAATGTGTGGAAACATGGATTTCTTCTC
TTCTGGATCACTTGGTGAAGTTGATTTCTGTCCTGTTCCACAAGCTGAGCCTGATTCCAT
TGTTGAAGATGACTATACTGATGATGAGATTGATGTTGATGAATTGGAGAGGAGGATGTG
GAGAGACAAAATGCGGCTTAAACGTCTCAAGGAGCAGGATAAGGGTAAAGAAGGTGTTGA
TGCTGCTAAACAGAGGCAGTCTCAAGAGCAAGCTAGGAGGAAGAAAATGTCTAGAGCTCA
AGATGGGATCTTGAAGTATATGTTGAAGATGATGGAAGTTTGTAAAGCTCAAGGCTTTGT
TTATGGGATTATTCCGGAGAATGGGAAGCCTGTGACTGGTG

Last Updated on 06.12.2018 16:11