Sequences from confirmation amplicon


Confirmation sequences for line 393A09 and hit At2g26310 are shown.
Line ID 393A09
Locus At2g26310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11203566
Primers used for PCR BD13 - 8474, show primer details
   
Sequence Name 26-K028145-022-393-A09C-8409
GenBank Accession LN497777 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K028145-022-393-A09C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGGACAATAATAGACTT
TCGGCGAACAGAAGATGGACAGCTAATCACAGAGAGTGAGTAATAATTATGAAGCTTCCT
TCTCTATGCAATTTATTGTATACCTGACCTGAACATGGCCAACGTTTGCAGTTGGTGGTA
ACCTGATTGGAGCTGTCCGAAGCAAGGATCTTTGCAGTTAGTTCTTCATTCTAATTATTT
GTTTTCTCGTTTTTGTTTGCGTACTAAAAGTCCAAGGGCTCTGAGATTTGTGTACATTGC
AGGAGCATTTTTTGGTATGTACATTGGAGATGTTCCGGTGTCAGAGCAGACAAAGGAGGA
GATTGGTAGAAAAGTGGTGGGAATCATAAAGAGATGCTGAAATCAATTACTTGTTAGATT
TCCAATTGCCTCTGAGTTTTAGAAGAAGGCAATTGGGTTAGATTTACGCGGTCATGTCTA
GATATTGTCAATCAAAATACTTTGGAAGCAGCTTGGAGGAAGTTATGTCTTAAGTTGTAT
TACATAGTCTTGATTTTTATGTTCTAGTTCGTTATG
   
Sequence Name 27-K028145-022-393-A09C-BD13
GenBank Accession LN498241 [GenBank]
Sequencing primer gene specific primer, BD13: 5'-GATCCAAGAAGAGCAACAAAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K028145-022-393-A09C-BD13
TTATTTCATTTAGAGATGGAGAGTTTATTTGTACTTAGCCGACTGAAGATCATAGGAAAT
CATGTTCTATTTTCTTCAAACGTTTCATATGTAATCATTAATTGAATTTGAAATTTTGTT
TTATATTTTTTAATATTAAATGTTGGATCTCATTTCTTGTCAACCAGTTTCACAATTTGC
ATATTGGACAATCATAACGAACTAGAACATAAAAATCAAGACTATCTAATACAACTTAAG
ACATAACTTCCTCCAAGCTGCTTACAAAGTATTTTGATTGACAATATCTAGACATGACCG
CGTAAATCTAACCCAATTGCCTTCTTCTAAAACTCAGAGGCAATTGGAAATCTAACAAGT
AATTGATTTCAGCATCTCTTTATGATTCCCACCACTTTTCTACCAATCTCCTCCTTTGTC
TGCTCTGACACCGGAACATCTCCAATGTACATACCAAAAAATGCTCCTGCAATGTACACA
AATCTCAGAGCCCTTGGACTTTTAGTACGCAAACAAAAACGAGAAAACAAATAATTAGAA
TGAAGAACTAACTGCAAAGATCCTTGCTTCGGACAGCTCCAATCAGGTTACCACCAACTG
CAAACGTTGGCCATGTTCAGGTCAGGTATACAATAAATTGCATAGAGAAGGAAGCTTCAT
AATTATTACTCACTCTCTGTGATTAGCTGTCCATCTTCTGTTCGCCGAAAGTCTATT

Last Updated on 06.12.2018 16:11