Sequences from confirmation amplicon


Confirmation sequences for line 393B06 and hit At1g27130 are shown.
Line ID 393B06
Locus At1g27130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9426565
Primers used for PCR BG66 - 8474, show primer details
   
Sequence Name 14-K104021-0022-393-B06C-BG66
GenBank Accession LN492483 [GenBank]
Sequencing primer gene specific primer, BG66: 5'-TGAGGTTCAACTAAAAGCTTGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K104021-0022-393-B06C-BG66
ATTAGATCCGATTTACGGATATGGGTATTTTGATTAATCTGTGTTTGCTTGCTAAAGTTT
GAAACTTTTTAGATGGAGCTAAACAAAAAATACTCTGTTCTTGATAGATCTGTGTAGCTT
GAGATCCGATTTATACACTCAACGCAGAAGTCTCTAGATTAGTCGTCTCAAGGGTTTTGT
TTTATGTTTATGAAACAGTGTTTTGCGGCGGTGGATGCGGTGGTCGGACCAAAAGACGAC
GAAGGGAAAATGGCGGCGGTGGGAAAGCTCATGGACTGTTTGGCGATACTTGAAGCATCG
TTTCACAAGAGCAGCAAAGGATCAGGGTTCTTTGGAGGACAGACCATCAGCTACCTTG
   
Sequence Name 30-K104022-0022-393-B06C-8409
GenBank Accession LN499692 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K104022-0022-393-B06C-8409
TTTACAATTGAATATATCCCGGACAACGACCTCTTCGACGGTTGGCATGTAGGGCTTAAC
AGCTTCATGGGCCCTAAACCTCTCGGCCCATTTGATCAACCCAGGTGTTGTCTCTTGCCG
GAGAAACTTGACGCCGGAAAACGCCTCGATCACAGAGATCGGACCTAAAAGAGCCGAACA
AGCAATGTCAAGGTAGCCGATGGTCTCTCCTCCAAAGAACCCTAATCCTTTGCTGCTCTT
CTGAAACGTTTCTTCAAGTATCGCCAAACACTCCATCAGCTTTCCCACCGCCGCCATTTT
CCCTTCGTCGTCTTTTGCTCCGACCACCGCATCCACCGCCGCAAAACACTGTTTCATAAA
CATAAAACAAAACCCTTGAGATGACTAATCTAGAGACTTCTGCGTTGAGTGTATAAATCG
GATCTCAAGCTACACAGATCTATCAAGAACAGAGTATTTTTTGTTTAGCTCCATCTAAAA
AGTTTCAAACTTTAGCAAGCAAACACAGATNAATCAAAATACCCATATCCGTAAATCGGA
TCTTAATCAGTCAGATCTATATNTGAGTGTCAAGATCCAAGCTTTTAGTTGAACCCTC

Last Updated on 06.12.2018 16:11