Sequences from confirmation amplicon


Confirmation sequences for line 393C06 and hit At2g04270 are shown.
Line ID 393C06
Locus At2g04270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:1476017
Primers used for PCR JV54 - 8474, show primer details
   
Sequence Name 09-K101423-0022-393-C06C-8409
GenBank Accession LN490112 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K101423-0022-393-C06C-8409
GCCATTTACATCCGATGAAGATCCATATCCAGCTTTTAACAGATAATTATACCTGAAATT
TACACCAGAAGCAATCTGCCAAGCATATTAAGTTAAAAGCAAACACGACATCTGAACTCA
CTTTCAAGAATTGAGGTTGGTTCCTTTTGTTACTACGACTAAACGTTCAAACGTTTTATG
TAAATTAAGATACATCTCTACTTATGTTCGCTACGGCTTAAGTGAAACAAAAGAATAGAT
TACCTTGACTTTAGCTTCCCATTCATTATCATTTTCCGTAGGATACATTGAGATTGCGCA
GTCTGGCTCCCAGCTACCTAATGTAGAAGGATCTCCAGTAACGTAGAGATGTTCATTGGC
TGCCAGATCAGCTTCCACAATCCAAACGACCTCGCATAATCCTTTGAGACGA
   
Sequence Name 49-K101426-0022-393-C06C-JV54
GenBank Accession LN506778 [GenBank]
Sequencing primer gene specific primer, JV54: 5'-CCTAGTCGTCTCAAAGGATTATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K101426-0022-393-C06C-JV54
ATCTGGCAGCCATGAACATCTCTACGTTACTGGAGATCCTTCTACATTCGGTAGCTGGGA
GCCAGACTGCGCAATCTCAATGTATCCTACGGAAAATGATAATGAATGGGAAGCTAAAGT
CAAGGTAATCTATTCTTTTGTTTCACTTAAGCCGTAGCGAACATAAGTAGAGATGTATCT
TAATTTACATAAAACGTTTGAACGTTTAGTCGTAGTAACAAAAGGAACCAACCTCAATTC
TTGAAAGTGAGTTCAGATGTCGTGTTTGCTTTTAACTTAATATGCTTGGCAGATTGCTTC
TGGTGTAAATTTCAGGTATAATTATCTGTTAAAAGCTGGATATGGATCTTCATCGGATGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11