Sequences from confirmation amplicon


Confirmation sequences for line 396D09 and hit At4g02420 are shown.
Line ID 396D09
Locus At4g02420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1064441
Primers used for PCR EQ43 - 8474, show primer details
   
Sequence Name 32-K103006-0022-396-D09C-8409
GenBank Accession LN500537 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K103006-0022-396-D09C-8409
TCTCCCTTAGATACTGTAGCACTTGTCTCATTGTTGGTCTAGCTAGTGGGTCGGAGTGAG
AGCACAACATACCTAGCTTCAAAACCATTTCGACTTCTTTTTGGTCATACTCAGACCCTA
GATTAGGATCCTTAGCATCCAAAATGTTTGCCTCCATCCAAAATCTAAAAACCCAATCCA
CGAGCACGACCCTCTCACCACTTTGGTTATTAATCTCGATAGGACGTCTGCCGCACGCCA
CTTCTAGTAGGAGCACCCCAAATGCAAAAACATCAGTAGTGGTTGTGGCCCGTCCTGTCC
TGATGTGATCGGGGGCTAGGTATCCCCAAGTTCCAACTACACGCGTAGTTTGAGGATCAG
ACCCGTGATCACACAACTGAGCTAAACCGAAATCCCCGAGTCTCCCATTGAGCTCTGCAT
CTAACAAGACGTTGCTGGCTTTGACGTCGCGGTGAATCACCACTTGTTCCCACTCCTCGT
GGAGATAAAATAAGGCAGAGGCCACGCCATTAATGACTTTAAACCTCTGTTTCCAATCGA
GGGTTACCTCTGGACTATNGTACAAATACTTGTCTAAACTTCCATTGGGCATGTAA
   
Sequence Name 80-K103007-0022-396-D09C-EQ43
GenBank Accession LN513937 [GenBank]
Sequencing primer gene specific primer, EQ43: 5'-ATTACATGCCCAATGGAAGTTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K103007-0022-396-D09C-EQ43
TACCCTCGATTGGAAACAGAGGTTTAAAGTCATTAATGGCGTGGCCTCTGCCTTATTTTA
TCTCCACGAGGAGTGGGAACAAGTGGTGATTCACCGCGACGTCAAAGCCAGCAACGTCTT
GTTAGATGCAGAGCTCAATGGGAGACTCGGGGATTTCGGTTTAGCTCAGTTGTGTGATCA
CGGGTCTGATCCTCAAACTACGCGTGTAGTTGGAACTTGGGGATACCTAGCCCCCGATCA
CATCAGGACAGGACGGGCCACAACCACTACTGATGTTTTTGCATTTGGGGTGCTCCTACT
AGAAGTGGCGTGCGGCAGACGTCCTATCGAGATTAATAACCAAAGTGGTGAGAGGGTCGT
GCTCGTGGATTGGGTTTTTAGATTTTGGATGGAGGCAAACATTTTGGATGCTAAGGATCC
TAATCTAGGGTCTGAGTATGACCAAAAAGAAGTCGAAATGGTTTTGAAGCTAGGTTTGTT
GTGCTCTCACTCCGACCCACTAGCTAGACCAACAATGAGACAAGTGCTACAGTATCTAAG
GGGAGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
ATT
Primers used for PCR KF54 - 8474, show primer details
   
Sequence Name 50-K118780-0022-396-D09C-KF54
GenBank Accession LN507326 [GenBank]
Sequencing primer gene specific primer, KF54: 5'-ATTGGTCCTCCAATGATATAGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K118780-0022-396-D09C-KF54
GTCGCAGCGTTATAAACCACCTTGGTTAATTAGACAATTTTAATTTATTTTTTTGCTTCT
TTTCATTAGGATTCTCTTAAGAAACTTGTAAGGTCTCTGCAATGGACATGCTCTTAGGAA
ACACTATTAATCGAAAAGCCATGTGATACATAATTATACTTCGATAACCATACACTTTCA
ATGCAGCAACAATAAAACTAAACGTTAGAGTTTCAAATATTTCACGTTTGAGTTCATGGT
CCTCTATCTTTTGCAGATAATTGCTTTCTACAAACGTTATGGTATACGAAAATCACCTCC
CACTGGAGAGTAGAGAATAAGCAACAGAAGATCCACTGGTAAACATGCCCGACTCATTAG
ACCCGTTGTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 10-K118779-0022-396-D09C-8409
GenBank Accession LN490882 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118779-0022-396-D09C-8409
GCATTTCATTGAATATTCCACACGGGTCTAATGAGTCGGGCATGTTTACCAGTGGATCTT
CTGTTGCTTATTCTCTACTCTCCAGTGGGAGGTGATTTTCGTATACCATAACGTTTGTAG
AAAGCAATTATCTGCAAAAGATAGAGGACCATGAACTCAAACGTGAAATATTTGAAACTC
TAACGTTTAGTTTTATTGTTGCTGCATTGAAAGTGTATGGTTATCGAAGTATAATTATGT
ATCACATGGCTTTTCGATTAATAGTGTTTCCTAAGAGCATGTCCATTGCAGAGACCTTAC
AAGTTTCTTAAGAGAATCCTAATGAAAAGAAGCAAAAAAATAAATTAAAATTGTCTAATT
AACCAAGGTGCGAACCTTAAATAAGGTATTTTTGGGTGAGTAAGGTTCGCTGACGTGGCA
CTATATCATTGGAGGACCAA

Last Updated on 06.12.2018 16:11