Sequences from confirmation amplicon


Confirmation sequences for line 398G03 and hit At1g64440 are shown.
Line ID 398G03
Locus At1g64440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23938243
Primers used for PCR ZZ38 - 8474, show primer details
   
Sequence Name 51-K020244-022-398-G03C-8409
GenBank Accession LN507362 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K020244-022-398-G03C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATACAAGGAACCTCCTT
TGGCCATTTACAATCAAATCTACAATAAAAGCTTCCACTTTGCTATCGAAAACGACTGAG
TACCTTTGTCCGTCCATAAGGACTCATTCCAGACAGGGGAGACTCTTCTGTACAAGGAAC
CTCCTTTGGCCAGCCATACACAGTAGCGGACGAAGAAAATACAAGCTGATTGAGATCAAT
GAGAGAAAGTTAAATCATGTTTCACTGTCACATACTCACATACATAAGGAATACCAAAAA
TCTGCAATACAGACCAAAAGGGGATTTTACCTTTTTACATCCGTGTGCAGCCATTACTTC
CAAAAGTGTAATAGTCGCAATCAAGTTATTGTTATAATACAGAAGTGGTTTCGCCACGCT
CTCACCAACTGCTTTCAATCCAGCAAAATGCATTACTGCATCAAACCTAGATTACAGCCA
CATAGAGCACTAAATCACATCTCTGGAGGACTGGAAACAAGAAGCATTTCACTGTAGATT
CAGATCTTAGCACGAAACATACAAAACTAATTTCCCTGTCCTCTCTTCCTAGGCTTTTCG
GATCAACATATATTATCATAAGGTCTATTAAGAATACGAAATGAAAGTCAAAATTCATGA
GATTTAAACTTGAACATGTTTTTGCGAGGAAATGAGTATATCAGTAAGAAGAGGCTACTA
CAAAACTCACTTTGTTTCGGAGAAAACCTTCTCAGTGCGGGT
   
Sequence Name 52-K020244-0022-398-G03C-ZZ38
GenBank Accession LN507679 [GenBank]
Sequencing primer gene specific primer, ZZ38: 5'-TGTATCTCGATTTGCTTACAATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K020244-0022-398-G03C-ZZ38
TATGTTTGATCAAATTGCTTTTAGTCGCAGTGGATTTCTGGATCAAATTGTGTTCGAGAT
TATCGTTAATCTGAGATTTTTGTTTCTGCAAATGTTAGATGGCTAATGTATCTTATACCT
GCAGGTGGACCTTCGCGATAAACCCGCACTTGAGAAGGTTTTCTCCGAAACAAAGTGAGT
TTTGTAGTAGCCTCTTCTTACTGATATACTCATTTCCTCGCAAAAACATGTTCAAGTTTA
AATCTCATGAATTTTGACTTTCATTTCGTATTCTTAATAGACCTTATGATAATATATGTT
GATCCGAAAAGCCTAGGAAGAGAGGACAGGGAAATTAGTTTTGTATGTTTCGTGCTAAGA
TCTGAATCTACAGTGAAATGCTTCTTGTTTCCAGTCCTCCAGAGATGTGATTTAGTGCTC
TATGTGGCTGTAATCTAGGTTTGATGCAGTAATGCATTTTGCTGGATTGAAAGCAGTTGG
TGAGAGCGTGGCGAAACCACTTCTGTATTATAACAATAACTTGATTGCGACTATTACACT
TTTGGAAGTAATGGCTGCACACGGATGTAAAAAGGTAAAATCCCCTTTTGGTCTGTATTG
CAGATTTTTGGTATTCCTTATGTATGTGAGTATGTGACAGTGAAACATGATTTAACTTTC
TCTCATTGATCTCAATCAGCTTGTATTTTCTTCGTCCGCTACTGTGTATGGCTGGCCA

Last Updated on 06.12.2018 16:11